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Development of a Validated RP-HPLC Method for the Estimation of Curcumin in Nanoemulsion and in Its Phase Solubility Studies
Abstract
The study developed a simple, sensitive and robust method for the estimation of curcumin in bulk drug form and in nanoemulsion formulation. Mobile phase consisting of acetonitrile and 20 mM potassium dihydrogen orthophosphate (pH 3.0) in ratio 55:45 was selected. A flow rate of 1 mL/min and an injection volume of 20 μL was injected to develop a chromatogram using a 516 C18 DB reversed-phase column. The detection wavelength was adjusted at 420 nm and the retention time of curcumin was found to be 9.78±0.5 min. The method was validated as per ICH guidelines and can be used for the quantification of curcumin even at a very low concentration.
About the Authors
Z. Naz
Jamia Hamdard;
College of Pharmacy, Al Asmarya University
India
India
New Delhi, Zliten
M. S. Faisal
College of Pharmacy, Al Asmarya University
Libya
Libya
Zliten
A. B. Khan
Jamia Hamdard
India
India
New Delhi
A. Naz
Jamia Hamdard
India
India
New Delhi
F. J. Ahmad
Jamia Hamdard
India
India
New Delhi
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Review
For citations:
Naz Z., Faisal M.S., Khan A.B., Naz A., Ahmad F.J. Development of a Validated RP-HPLC Method for the Estimation of Curcumin in Nanoemulsion and in Its Phase Solubility Studies. Zhurnal Prikladnoii Spektroskopii. 2023;90(6):974.
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