DETERMINATION OF CERIUM (IV) USING RHODAMINE 6G FLUORESCENCE QUENCHING

Abstract

The interaction between rhodamine 6G (Rh6G) and cerium sulfate was studied by the fluorescence quenching method. In a sulfuric acid medium, the interaction of Ce(IV) with Rh6G results in Rh6G fluorescence quenching. The maximum excitation wavelength (l_ex) and the maximum emission wavelength (l_em) are 530 nm and 555 nm, respectively. A good linearity between the relative fluorescence intensity (ΔF) and Ce(IV) was observed in the range ~0.12-1.08 mg/mL. The detection limit was 1.4´10^-3mg/mL. The optimum reaction conditions, influencing factors, and effect of coexisting substances were investigated in the experiment. We found that the concentration of Rh6G was 3.2´10^-6 mol/L, and the fluorescence intensity was maximum.