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Rapid Discrimination of Cervical Cancer from Hysteromyoma Using Label-Free Serum RNA Based on Surface-Enhanced Raman Spectroscopy and AdaBoost Algorithm
Abstract
We investigated the feasibility of using surface-enhanced Raman scattering (SERS) technology combined with the AdaBoost algorithm to rapidly discriminate cervical cancer patients from hysteromyoma patients. Using Au colloids as the SERS active substrate, we recorded Raman signal measurements on serum RNA samples obtained from 35 patients diagnosed with cervical cancer and 30 patients diagnosed with hysteromyoma. Analysis of RNA SERS spectra using principal component analysis, then three principal components (PC2, PC11, and PC24) with significant differences were chosen using the independent samples t-test (p < 0.05). The distinctive peak intensities of the relevant substance, measured at 448, 519, 698, 1003, and 1076 cm–1 , were found to be correlated with the substance's alterations during the carcinogenesis process. The ideal AdaBoost classification model was developed by fine-tuning its parameters. The model showcased an impressive accuracy of 96.92%, exhibiting a high sensitivity of 94.28% and an exceptional specificity of 100%, as reported in the results. Compared to the linear discriminant analysis, support vector machine models, the effectiveness of classification greatly improved. The current findings indicate that serum SERS technology, combined with the AdaBoost algorithm, is anticipated to be developed into a potent screening tool for cervical cancer.
Supporting agencies: This research was supported by the National Natural Science Foundation of China (No. 62071059), the Prevention and Treatment of Central Asian High Incidence Diseases Fund (SKL-HIDCA-2019-5), the Science and Technology Department of Xinjiang Uyghur Autonomous Region (No. 2019E0282), and the Fundamental Research Funds for the Central Universities (No. 500422107).
About the Authors
Z. Jiao
School of Science, Beijing University of Posts and Telecommunications
China
China
Beijing
G. Wu
School of Electronic Engineering, Beijing University of Posts and Telecommunications
China
China
Beijing
J. Wang
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Gynecology, The First Affiliated Hospital of Xinjiang Medical University
China
China
Urumqi
X. Zheng
School of Electronic Engineering, Beijing University of Posts and Telecommunications
China
China
Beijing
L. Yin
School of Electronic Engineering, Beijing University of Posts and Telecommunications
China
China
Beijing
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For citations:
Jiao Z., Wu G., Wang J., Zheng X., Yin L. Rapid Discrimination of Cervical Cancer from Hysteromyoma Using Label-Free Serum RNA Based on Surface-Enhanced Raman Spectroscopy and AdaBoost Algorithm. Zhurnal Prikladnoii Spektroskopii. 2024;91(1):166.
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