Spectral Properties of Biodyes in the Formation of New Preparative Forms of Prodrugs

Abstract

The spectral and bactericidal properties of a number of biodyes such as bromophenol blue, amido black 10 B, methyl thymol blue, basic fuchsin, chrome dark blue, eosin, dye (indigo), bromophenol red were characterized. It has been shown that the inclusion of eosin, bromophenol red, indigo and rifampicin into the internal space of phospholipid nanocontainers leads to leveling of the characteristic absorption maxima in the visible or ultraviolet region for the spectra of these compounds in the form of an aqueous solution. This allows visual and spectrophotometric monitoring of the process of the prodrug incorporation into liposomes. The construction of a number of supramolecular forms of prodrugs, which are micelles and liposomes from modified forms of the bactericidal nucleoside brivudine in various combinations, showed the feasibility of using indigo as a dye marker. This made it possible to identify liposomes formed from an equimolar mixture of dimyristoylphosphatidylbrivudine lipoconjugate with dimyristoylphosphatidylcholine as a potential prodrug formulation that most effectively inhibits the growth of Staphylococcus aureus cells.