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Spectroscopic Studies on Plant Extract Mediated ZnO Nanoparticles as a Potential Cytotoxic Agent
Abstract
Plants play an important role in nanoparticle preparation because they are easily accessible, environmentally friendly, and inexpensive. In this study, we used an ethanolic extract of Mangifera indica seed as a reducing and stabilising agent to create zinc oxide (ZnO) nanoparticles (NPs). The ZnO NPs were examined using characterization techniques such as UV-Vis, Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The interaction of phytochemical constituents from plant extracts providing the biological reduction of zinc metal ions to ZnO had been identified by the UV-visible absorption studies. According to the FT-IR results, metal oxides exhibited interatomic vibration-driven absorption in the fingerprint area below 1000 cm–1. Particles appeared to be crystalline and also a rice-grain shape of ZnO NPs was confirmed by XRD, SEM, and TEM, respectively. In addition, the cytotoxic effect of ZnO NPs was checked using the SKMEL-28 cell line, showing an IC50 value of 32.686 μg/mL in the SKMEL-28 cell line, and 49.011 μg/mL in the typical L6 cell line. Furthermore, the synthesized NPs were subjected to (AO/EB) double staining approach to examine the apoptotic activity. The acridine orange/ethidium bromide method made strong evidence for demonstrating chromatin condensation and membrane blebbing.
About the Authors
B. T. Delma
Department of Chemistry, Holy Cross College (Autonomous) (Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli, Tamil Nadu, India)
India
India
Nagercoil, Tamil Nadu
M. Antilin Princela
Department of Chemistry, Holy Cross College (Autonomous) (Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli, Tamil Nadu, India)
India
India
Nagercoil, Tamil Nadu
Y. Subbareddy
Department of Chemistry, Andhra Loyola College (Autonomous)
India
India
Vijayawada
M. Anitha Malbi
Department of Chemistry, Holy Cross College (Autonomous) (Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli, Tamil Nadu, India)
India
India
Nagercoil, Tamil Nadu
S. Lizy Roselet
Department of Chemistry, Holy Cross College (Autonomous) (Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli, Tamil Nadu, India)
India
India
Nagercoil, Tamil Nadu
M. Shirly Treasa
Department of Chemistry, Holy Cross College (Autonomous) (Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli, Tamil Nadu, India)
India
India
Nagercoil, Tamil Nadu
M. C. Rao
Department of Physics, Andhra Loyola College (Autonomous)
India
India
Vijayawada
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Review
For citations:
Delma B.T., Princela M.A., Subbareddy Y., Malbi M.A., Roselet S.L., Treasa M.Sh., Rao M.C. Spectroscopic Studies on Plant Extract Mediated ZnO Nanoparticles as a Potential Cytotoxic Agent. Zhurnal Prikladnoii Spektroskopii. 2024;91(4):611.
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