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Photophysical Features of Protolytic Equilibria of Fluorescein Bifluorophores
Abstract
The photophysical properties of these two carboxyfluorescein bifluorophores were investigated in more depth, including the effects of pH and different solution media on the photophysical properties, as well as comparing the fluorescence effect of bifluorophores with monomers carboxyfluoresceins (5-FAM and 6-FAM) in conjugates with protein molecules. The results showed that both bifluorophores fluoresced perfectly in alkaline medium, but the fluorescence intensity decreased at pH values of 6–7, and almost disappeared in acidic medium. The fluorescence of (6-FAM)2 is very sensitive to the solution composition, while (5-FAM)2 is more stable and almost independent of the solution medium. When bifluorophore-protein conjugate is formed, the quantum yield of bifluorophore fluorescence decreases more strongly compared to monomers carboxyfluoresceins, but the overall fluorescence intensity of the bifluorophore was not inferior to that of the two carboxyfluorescein monomer-protein conjugates due to the high extinction coefficient of the bifluorophore (~140,000 M–1 . cm–1).
Supporting agencies: The work was carried out with the financial support of the China Scholarship Council Foundation (grant No. 202008400011), the Belarusian Republican Foundation for Basic Research (grants No. F24B-006 and No. X23RNF-041), Russian Science Foundation (grant No. 23-45-10010)
About the Authors
F. Fan
Institute of Physical-Organic Chemistry of the National Academy of Sciences
Belarus
Belarus
Minsk
V. A. Povedailo
B. I. Stepanov Institute of Physics of the National Academy
of Sciences of Belarus
Belarus
Belarus
Minsk
I. L. Lysenko
Institute of Physical-Organic Chemistry of the National Academy of Sciences
Belarus
Belarus
Minsk
O. L. Sharko
Institute of Physical-Organic Chemistry of the National Academy of Sciences
Belarus
Belarus
Minsk
I. O. Mazunin
Center for Molecular and Cellular Biology of the Skolkovo Institute of Science and Technology
Russian Federation
Russian Federation
Moscow
S. A. Tikhomirov
B. I. Stepanov Institute of Physics of the National Academy
of Sciences of Belarus
Belarus
Belarus
Minsk
V. V. Shmanai
Institute of Physical-Organic Chemistry of the National Academy of Sciences
Belarus
Belarus
Minsk
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Review
For citations:
Fan F., Povedailo V.A., Lysenko I.L., Sharko O.L., Mazunin I.O., Tikhomirov S.A., Shmanai V.V. Photophysical Features of Protolytic Equilibria of Fluorescein Bifluorophores. Zhurnal Prikladnoii Spektroskopii. 2024;91(5):723-732. (In Russ.)
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