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Derivative UV-Spectrophotometric Assay Methods for Determination of Ivacaftor
Abstract
Ivacaftor is a selective small molecule potentiator Transmembrane Conductance Regulator protein used for the treatment of cystic fibrosis. This report describes the validation of simple, rapid, sensitive and cost-effective zero-order and first-order derivative spectrophotometric methods for the estimation of ivacaftor in bulk and in its marketed formulation. Preliminary spectrophotometric analysis of the drug was carried out in methanol and a total of 21 parametric variations were considered. Three selected method variants employing absorbance (zero-order), peak-peak (first order) and peak-zero (second order) techniques were assessed for their stability indicating potential in stress degraded solutions of the drug. The developed method was validated with respect to parameters including linearity, accuracy, precision, robustness and solution stability. Excellent linearity was observed in the concentration range of 5.0–30.0 μg/mL with a correlation coefficient of 0.99 and above for the selected method variants. The limits of assay detection values ranged from 0.90–1.06 μg/mL, and quantitation limits ranged from 2.72–3.22 μg/mL for the proposed method variants. The proposed methods were used to quantify the drug in its marketed tablet formulation, and good recoveries ranging from 95.98 to 98.81 % were obtained.
Supporting agencies: The research grant provided by the University Grants Commission (UGC) and financial assistance from FIST grant of Department of Science & Technology (DST) is duly acknowledged
About the Authors
A. Bishnoi
Panjab University
India
India
Aastha Bishnoi
University Institute of Pharmaceutical Sciences; UGC Center of Advanced Study
Chandigarh
A. Bali
Panjab University
India
India
Alka Bali
University Institute of Pharmaceutical Sciences; UGC Center of Advanced Study
Chandigarh
A. Kumar
Panjab University
India
India
Akshay Kumar
University Institute of Pharmaceutical Sciences; UGC Center of Advanced Study
Chandigarh
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Review
For citations:
Bishnoi A., Bali A., Kumar A. Derivative UV-Spectrophotometric Assay Methods for Determination of Ivacaftor. Zhurnal Prikladnoii Spektroskopii. 2024;91(5):760.
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