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Evaluation of stability constant for vit В1 depending on stoichiometric curves by visible spectroscopy
Abstract
The measurement of vitamin B1 in pure and pharmaceutical formulations was proposed by using a straightforward and sensitive spectrophotometric approach. Sulfacetamide (SFA) is diazotized, then coupled with vitamin B1 in alkaline media to produce a coloured azo dye complex with a stability constant of 5.597×105 L/mol. The product is stable, with a maximum absorption wavelength of 489.5 nm, molar absorptivity of 10108 L/mol ∙ cm, Sandell’s sensitivity of 0.0334 μg/cm2, detection limit of 0.0135 μg/mL, and Beer's law being observed over the concentration range of 0.2–20.0 μg/mL. The stability constant and stoichiometry of the produced azo dye were calculated using the continuous variation (Job’s) and mole ratio methods. The suggested approach was used successfully in the determination of vitamin B1 in its pharmaceutical formulations.
About the Authors
Azhar S. Hamody
Department of Chemistry, College of Education for Pure Science Ibn Al-Haitham, Adhamiya, University of Baghdad
Iraq
Iraq
Baghdad
Nahla A. Alassaf
Department of Chemistry, College of Education for Pure Science Ibn Al-Haitham, Adhamiya, University of Baghdad
Iraq
Iraq
Baghdad
Resala A. Saleh
Department of Chemistry, College of Education for Pure Science Ibn Al-Haitham, Adhamiya, University of Baghdad
Iraq
Iraq
Baghdad
Faeza H. Zankanah
Department of Chemistry, College of Education for Pure Science Ibn Al-Haitham, Adhamiya, University of Baghdad
Iraq
Iraq
Baghdad
Sarmad B. Dikran
Department of Chemistry, College of Education for Pure Science Ibn Al-Haitham, Adhamiya, University of Baghdad
Iraq
Iraq
Baghdad
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Review
For citations:
Hamody A.S., Alassaf N.A., Saleh R.A., Zankanah F.H., Dikran S. Evaluation of stability constant for vit В1 depending on stoichiometric curves by visible spectroscopy. Zhurnal Prikladnoii Spektroskopii. 2024;91(6):914.
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