Development and validation of ultraviolet spectroscopic method for estimation of voriconazole in bulk and pharmaceutical formulation

Abstract

The present work aimed to develop a simple analytical method and validate the same for the estimation of Voriconazole using ultraviolet spectrophotometry. Methanol and artificial vaginal fluid (AVF) pH 4.1 were chosen as solvents for the development of the analytical method and the validation of the method was done for the different parameters. Voriconazole showed higher absorbance at 256 nm both in methanol and AVF pH 4.1. Linearity, precision, and accuracy as the analytical parameters were studied as per the International Conference of Harmonization (ICH) guidelines. The concentration range of 10–50 μg/mL in methanol and AVF pH 4.1 showed a linear response and followed Beer’s law with coefficient correlation values of 0.998 for methanol and 0.999 for AVF pH 4.1. The results for the accuracy and the repeatability parameter presented values that lie within the range of 98–102%, indicating a higher confidence interval. Regarding the method's sensitivity, the LOD for the drug in methanol and AVF pH 4.1 was found to be 2.55 and 2.00 μg/mL, respectively. The LOQ for the drug in methanol was 7.75 μg/mL and in AVF pH 4.1 was  6.08 μg/mL. The proposed, developed and validated method was simple, sturdy, cost-effective, and timesaving. The method could be further applied to calculate the entrapment efficiency of the drug-loaded nanostructured lipid carrier and tablet drug content.