VISUALIZATION OF PORPHYRIN-BASED PHOTOSENSITIZER DISTRIBUTION FROM FLUORESCENCE IMAGES in vivo USING AN OPTIMIZED RGB CAMERA
Abstract
A handheld RGB camera was developed to monitor the in vivo distribution of porphyrin-based photosensitizer (PS) hematoporphyrin monomethyl ether (HMME) in blood vessels during photodynamic therapy (PDT). The focal length, f-number, International Standardization Organization (ISO) sensitivity, and shutter speed of the camera were optimized for the solution sample with various HMME concentrations. After the parameter optimization, it was found that the red intensity value of the fluorescence image was linearly related to the fluorescence intensity under investigated conditions. The RGB camera was then used to monitor the in vivo distribution of HMME in blood vessels in a skin-fold window chamber model. The red intensity value of the recorded RGB fluorescence image was found to be linearly correlated to HMME concentrations in the range 0-24 μM. Significant differences in the red to green intensity ratios were observed between the blood vessels and the surrounding tissue.
About the Authors
L. . Liu
Fujian Normal University
Russian Federation
Russian Federation
Zh. . Huang
Fujian Normal University
Russian Federation
Russian Federation
Zh. . Qiu
Fujian Normal University
Russian Federation
Russian Federation
B. . Li
Fujian Normal University
Russian Federation
Russian Federation
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Review
For citations:
Liu L., Huang Zh., Qiu Zh., Li B. VISUALIZATION OF PORPHYRIN-BASED PHOTOSENSITIZER DISTRIBUTION FROM FLUORESCENCE IMAGES in vivo USING AN OPTIMIZED RGB CAMERA. Zhurnal Prikladnoii Spektroskopii. 2017;84(6):1018(1)-1018(7). (In Russ.)
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