Preview

Zhurnal Prikladnoii Spektroskopii

Advanced search
Open Access Open Access  Restricted Access Subscription Access

UV Spectroscopy-Based Development and Validation of an Analytical Method for Simultaneous Estimation of Ellagic Acid and Quercetin in Bulk Suspension

Abstract

А UV visible spectroscopic method has been developed for the simultaneous estimation of quercetin and ellagic acid in a suspension. Validation of the developed method was done according to ICH Q2R2 guidelines. Calibration curves (R2>0.98) were prepared, demonstrating a linear response for the concentration ranges of both drugs. The results indicated high levels of accuracy and precision (all RSD% values <2%). The findings suggest that this method can be readily adopted in routine laboratory settings, facilitating the simultaneous monitoring of drug levels in both bulk and dosage forms.

About the Authors

K. Malhotra
Amity Institute of Pharmacy, Amity University Uttar Pradesh
India

Kabil Malhotra.

Noida U.P.



K. Nagpal
Amity Institute of Pharmacy, Amity University Uttar Pradesh
India

Kalpana Nagpal.

Noida U.P.



References

1. H. Kaur, A. Thakkar, K. Nagpal, J. Pharm. Technol., Research and Management, 7, 1–5 (2019).

2. S. W. Park, M. J. Kwon, J. Y. Yoo, H. J. Choi, Y. J. Ahn, BMC Complement Altern. Med., 14, 171 (2014), https://doi.org/10.1186/1472-6882-14-171

3. N. Srivastava, A. Bansal, K. Aggarwal, K. Nagpal, J. Appl. Spectrosc., 91, 700–708 (2024).

4. S. U. Mertens-Talcott, S. T. Talcott, S. S. Percival, J. Nutr., 133, 2669–2674 (2003).

5. K. L. Khanduja, R. K. Gandhi, V. Pathania, N. Syal, Food and Chem. Toxicology, 37, 313–318 (1999).

6. S. U. Mertens-Talcott, J. A. Bomser, C. Romero, S. T. Talcott, S. S. Percival, J. Nutrition, 135, 609–614 (2005).

7. D. B. Narayana, S. Durg, P. R. Manohar, et al., J. Ethnopharmacol., 197, 52–60 (2017), doi: 10.1016/j.jep.2016.07.078.

8. S. Ahmed, X. Ding, A. Sharma, J. Ethnopharmacol., 273, 113829 (2021).

9. R. Sharma, N. Martins, K. Kuca, A. Chaudhary, A. Kabra, M. M. Rao, P. K. Prajapati, Biomolecules, 9, 161 (2019).

10. H. N. A. Hassan, B. N. Barsoum, I. H. I. Habib, J. Pharm. Biomed. Anal., 20, 315–320 (1999).

11. I. Baranowska, D. Raróg, Talanta, 55, 209–212 (2001).

12. N. Pejic, V. Kuntic, Z. Vujic, S. Micic, Farmaco, 59, 21–24 (2004).

13. P. C. Morrice, S. G. Wood, G. G. Duthie, J. Chromatogr. B: Biomed. Sci. Appl., 738, 413–417 (2000).

14. R. Aguilar-Sánchez, F. Áhuatl-García, M. M. Dávila-Jiménez, M. P. Elizalde-González, M. R. G. Guevara- Villa, J. Pharm. Biomed. Anal., 38, 239–249 (2005).

15. P. I. D. Assunção, E. C. Conceição Da, L. L. Borges, J. A. M. Paula De, Evid. Based Complement. Alternat. Med., 2017, 1501038–1501038 (2017).

16. P. More, A. P. Jadhav, Indian Drugs, 58, 52–57 (2021).

17. S. Shaikh, V. Jain, International J. Appl. Pharm., 10, 111–116 (2018).

18. EPA ORD NCEA Integrated Risk Information System U (1999) Toxicological Review of Acetonitrile (CAS No. 75-05-8) (PDF).

19. J. Chaudhary, Int. Res. J. Pharm., 2, 111–113 (2011).

20. M. S. Vardhan, K. V. V. S. Krishna, K. G. Sankar, S. R. Krishna, T. T. Mani, Res. J. Pharm. Phytochem., 9, 223–227 (2017).

21. K. Shalini, K. Ilango, Int. J. Appl. Pharm., 13, 183–192 (2021).

22. S. Shaikh, V. Jain, Int. J. Appl. Pharm., 10, 169–174 (2018).

23. B. Cassidy, T. Bloomingdale, J. Carmody, J. Pharm. Sci., 117, No. 6, 103749 (2025); https://doi.org/10.1016/j.xphs.2025.103749

24. V. Pilařová, K. Plachká, L. Chrenková, I. Najmanová, P. Mladěnka, F. Švec, O. Novák, L. Nováková, Talanta, 185, 71–79 (2018).

25. C. E. Alupoaei, L. H. García-Rubio, Chem. Eng. Comm., 192, 198–218 (2005).

26. P. Li, J. Hur, Crit. Rev. Environ. Sci. Technol., 47, 131–154 (2017).


Review

For citations:


Malhotra K., Nagpal K. UV Spectroscopy-Based Development and Validation of an Analytical Method for Simultaneous Estimation of Ellagic Acid and Quercetin in Bulk Suspension. Zhurnal Prikladnoii Spektroskopii. 2025;92(4):560.

Views: 77


ISSN 0514-7506 (Print)