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“Protect-Deprotect” Based Fluorescent Probe for the Recognition of Norepinephrine
Abstract
Research has shown a significant correlation between severe depression and reduced levels norepinephrine (NE) in brain tissue. Therefore, designing NE-specific fluorescent probes is highly challenging. We adopted a “protect-deprotect” strategy in which the 7-hydroxy-4-methylcoumarin fluorescence group was protected by a carbonic ester that linked to the departing thiophenol group. The probe then underwent two nucleophilic additions and eliminations by NE, which released the fluorophore and enabled specific fluorescence detection of NE with sensitivity and selectivity. This recognition mechanism was verified using the highresolution mass-spectrometry method.
Keywords
norepinephrine,
nucleophilic additions,
“protect-deprotect” strategy,
fluorescent probe,
cell imaging
Supporting agencies: The work was supported by the Natural science fund (No. 23KJA350004) for colleges and universities in Jiangsu Province
About the Authors
Wei Gao
Changzhi University
China
China
Department of Chemistry
Changzhi
Hao Na Zhang
Yancheng Teachers’ University
China
China
School of Pharmacy
Yancheng
Wen Ling Wang
Yancheng Teachers’ University
China
China
School of Pharmacy
Yancheng
Yan Li Chen
Yancheng Teachers’ University
China
China
School of Pharmacy
Yancheng
Qingming Wang
Yancheng Teachers’ University
China
China
School of Pharmacy
Yancheng
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Review
For citations:
Gao W., Zhang H.N., Wang W.L., Chen Ya.L., Wang Q. “Protect-Deprotect” Based Fluorescent Probe for the Recognition of Norepinephrine. Zhurnal Prikladnoii Spektroskopii. 2026;93(1):150/1-150/6.
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