SPECTROSCOPIC STUDY OF THE BINDING OF NETROPSIN AND HOECHST 33258 WITH NUCLEIC ACIDS
Abstract
The interaction of the groove binding compounds - peptide antibiotic netropsin and fluorescent dye (bis-benzimidazole) Hoechst 33258 - with the double-stranded DNA and synthetic double-stranded polynucleotide poly(rA)-poly(rU) has been studied by spectrophotometry. The absorption spectra of these ligand complexes with the nucleic acids have been obtained. Spectral changes at the complexation of individual ligands with the mentioned nucleic acids reveal the similarity of binding of each of these ligands with both DNA and RNA. Based on the spectroscopic measurements, the parameters of netropsin and Hoechst 33258 binding with DNA and poly(rA)-poly(rU) - K and n, as well as the thermodynamic parameters DS, DG, and DH have been determined. It is found that the binding of Hoechst 33258 with both nucleic acids is accompanied by a positive change in enthalpy, while in the case of netropsin the change in enthalpy is negative. Moreover, the contribution of the entropy factor to the formation of the complexes is more pronounced in the case of Hoechst 33258.
About the Authors
P. O. Vardevanyan
Yerevan State University
Russian Federation
Russian Federation
M. A. Parsadanyan
Yerevan State University
Russian Federation
Russian Federation
A. P. Antonyan
Yerevan State University
Russian Federation
Russian Federation
V. G. Sahakyan
Yerevan State University
Russian Federation
Russian Federation
References
1. D. Rentzeperis, L. A. Marky, T. J. Dwyer, B. H. Gelerstanger, J. G. Pelton, D. E. Wemmer. Biochemistry, 34, N 9 (1995) 2937-2945
2. A. N. Lane, T. C. Jenkins. Q. Rev. Biophys., 33, N 3 (2000) 255-306
3. S. K. R. Gowda, B. B. Mathew, C. N. Sudhmani, H. S. B. Naik. Biomed. Biotechnol., 2, N 1 (2014) 1-6
4. T. H. Doung, K. Zakrzewska. J. Biomol. Struct. Dyn., 14, N 6 (1997) 691-701
5. Y.-Y. Fang, V. R. Morris, G. M. Lingani, W. M. Southerland. Open Conf. Proc. J., N 1 (2010) 157-163
6. Ю. С. Бабаян, Л. Е. Ксодо, Дж. Манзини. Биофизика, 33, N 4 (1988) 716-721
7. E. A. Lewis, M. Munde, S. Wang, M. Retting, V. Le, V. Machha, W. D. Wilson. Nucl. Аcid. Res., 39, N 22 (2011) 9649-9658
8. В. Зенгер. Принципы структурной организации нуклеиновых кислот, Москва, Мир (1987) 418-520
9. R. Palchaudhuri, P. J. Hergenrother. Curr. Opt. Biotechnol., 18, N 6 (2007) 497-503
10. K. Mišković, M. Bujak, M. B. Loncar, L. Glavas-Obrovac. Arh. Hig. Rad. Toxicol., 64, N 4 (2013) 593-602
11. T. Yamori, A. Matsunaga, S. Sato, K. Yamazaki, A. Komi, K. Ishizu, I. Mita, H. Edatsugi. Cancer Res., 59 (1999) 4042-4049
12. M. H. David-Cordonnier, M. P. Hildebrand, B. Baldeyrou, A. Lansiaux, C. Keuser. Eur. J. Med. Chem., 42 (2007) 752771
13. B. K. Bhuyan, K. A. Newell, S. L. Crampton, D. D. Von Hoff. Cancer Res., 42 (1982) 3532-3537
14. X. Wanga, N. Chub, N. Wanga, L. Q. Chao, C. Jianga, X. Wanga, T. Ikejima, M. Chenga. Bioorg. Med. Chem. Lett., 22 (2012) 6297-6300
15. M. Hirota, T. Fujiwara, S. Mineshita, H. Sugiyama, H. Teraoka. Int. J. Biochem. Cell. Biol., 39 (2007) 988-996
16. В. В. Костюков, М. П. Евстигнеев. Физика живого, 18, N 2, (2010) 34-40
17. B. Nguen, S. Neidle, D. W. Wilson. Acc. Chem. Res., 42 (2009) 11-21
18. N. N. Degtyareva, B. D. Wallace, A. R. Bryant, K. M. Loo, J. T. Petty. Biophys. J., 92 (2007) 959-965
19. X. Shi, R. B. J. Macgregor. Biophys. Chem., 125 (2007) 471-482
20. П. О. Вардеванян, М. А. Парсаданян, А. П. Антонян, С. Н. Акопян. Журн. физ. химии, 91, N 6 (2017) 1071-1073
21. D. R. Corey. J. Clin. Invest., 117 (2007) 3615
22. C. N. N’soukpoe-Kossi, C. Descoteaux, E. Asselin, H. A. Tajmir-Riahi, G. Berube. DNA Cell Biol., 27 (2008) 1
23. S. R. Kirk, Y. Tor. Bioorg. Med. Chem., 7 (1999) 1979
24. S. Fulle, H. Gohlke. J. Mol. Recognit., 23 (2010) 220
25. S. Nafisi, F. Manouchehri, M. Bonsaii. J. Photochem. Photobiol. B: Biol., 111 (2012) 27
26. D. Khaitan, M. E. Dinger, J. Mazar, J. Crawford, M. A. Smith, J. S. Mattick, R. J. Perera. Cancer Res., 71 (2011) 3852
27. Д. Фрайфельдер. Физическая биохимия, Москва, Мир (1980)
28. J. Lah, N. Carl, I. Drobnak, B. Šumiga, G. Vesnaver. Acta Chim. Slov., 53 (2006) 284
29. S. Y. Breusegem, F. G. Loontiens, P. Regenfuss, R. M. Clegg. Methods Enzyme J., 340 (2001) 212
30. I. H. Stockley. In “Stockley’s Drug Interactions”, 6th ed., London, Pharm. Press (2002) 1080-1081
31. L. Tabernero, N. Verdaguer, M. Coll, I. Fita, G. A. van der Marel, J. H. van Boom, A. Rich, J. Aymami. Biochemistry, 32, N 33 (1993) 8403-8410
32. P. O. Vardevanyan, A. P. Antonyan, M. A. Parsadanyan, K. V. Pirumyan, A. M. Muradyan, A. T. Karapetian. J. Biomol. Struct. Dyn., 25, N 6 (2008) 641-646
33. J. A. Parkinson, S. E. Ebrahimi, J. H. Mckie, K. T. Douglas. J. Biochem., 33 (1994) 8442-8452
Review
For citations:
Vardevanyan P.O., Parsadanyan M.A., Antonyan A.P., Sahakyan V.G. SPECTROSCOPIC STUDY OF THE BINDING OF NETROPSIN AND HOECHST 33258 WITH NUCLEIC ACIDS. Zhurnal Prikladnoii Spektroskopii. 2018;85(2):321-326. (In Russ.)
Views: 255
Email this article 