MOLECULAR SPECTROSCOPY EVIDENCE OF EFFECTIVE CONSTITUENTS IN THE BINDING OF Angelica sinensis TO BOVINE SERUM ALBUMIN: COMPARATIVE BINDING AND THERMODYNAMIC PROFILE
Abstract
Some bio-effective constituents, such as organic acids and phthalides, from the root of Angelica sinensis can be observed in the blood after oral intake. In this work, two organic acids, ferulic acid and anisic acid, and two phthalides, senkyunolide I and butylidenephthalide, were selected to investigate the interaction with bovine serum albumin systematically, using a fluorescence spectroscopic method under physiological conditions. The Stern-Volmer quenching constant, binding constant, and the corresponding thermodynamic parameters and binding numbers were measured. Synchronous fluorescence and three-dimensional fluorescence spectroscopy were also used. The results provided molecular spectroscopy evidence that some constituents of Angelica sinensis are absorbed and transported into the blood.
About the Authors
Zh. . Wang
Pharmacy College of Hubei University of Medicine; Key Lab of Wudang Characteristic Herbal Study in Hubei Province; Engineering Research Center for Herbal Utilization of Hubei Province
Russian Federation
Russian Federation
X. . Deng
Pharmacy College of Hubei University of Medicine; Key Lab of Wudang Characteristic Herbal Study in Hubei Province; Engineering Research Center for Herbal Utilization of Hubei Province
Russian Federation
Russian Federation
J. . Hou
Gansu University of Chinese Medicine
Russian Federation
Russian Federation
J. . Fan
Pharmacy College of Hubei University of Medicine; Key Lab of Wudang Characteristic Herbal Study in Hubei Province; Engineering Research Center for Herbal Utilization of Hubei Province
Russian Federation
Russian Federation
References
1. L. Z. Yi, Y. Z. Liang, H. Wu, D. L. Yuan, J. Chromatogr. A, 1216, 1991 (2009).
2. S. Y. Ou, K. C. Kwok, J. Sci. Food Agric., 84, 1261 (2004).
3. T. T. X. Dong, K. J. Zhao, Q. T. Gao, Z. N. Ji, T. T. Zhu, J. Li, R. Duan, A. W. H. Cheung, K. W. K. Tsim, J. Agric. Food. Chem., 54, 2767 (2006).
4. D. H. F. Mak, P. Y. Chiu, T. T. X. Dong, K. W. K. Tsim, K. M. Ko, Phytother. Res., 20, 561 (2006).
5. J. J. Beck, S. C. Chou, J. Nat. Prod., 70, 891 (2007).
6. Z. B. Dong, S. P. Li, A. Hong, Q. Zhu, J. Pharm. Biomed. Anal., 38, 664 (2005).
7. Y. L. Wang, Y. Z. Liang, B. M. Chen, Y. K. He, B. Y. Li, Q. N. Hu, Anal. Bioanal. Chem., 383, 247 (2005).
8. D. E. T. Golan, E. J. Armstrong, Principles of Pharmacology: the Pathophysiologic Basis of Drug Therapy, Lippincott Williams & Wilkins (2011) 33.
9. F. Moreno, M. Cortijo, J. Gonzalez-Jimenez, Photochem. Photobiol., 70, 695 (1999).
10. A. Papadopoulou, R. J. Green, R. A. Frazier, J. Agric. Food. Chem., 53, 158 (2005).
11. M. Homma, K. Oka, T. Yamada, T. Niitsuma, H. Ihto, N. Takahashi, Anal. Biochem., 202, 179 (1992).
12. M. E. Georgiou, C. A. Georgiou, M. A. Koupparis, Anal. Chem., 71, 2541 (1999).
13. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Springer, 278-289 (2006).
14. K. A. Connors, Binding Constants: the Measurement of Molecular Complex Stability, Wiley, New York 31-75 (1987).
15. A. S. Ladokhin, Fluorescence Spectroscopy in Peptide and Protein Analysis, Wiley, New York (2006).
16. U. Kragh-Hansen, Pharmacol. Rev., 33, 17 (1981).
17. N. Tayeh, T. Rungassamy, J. R. Albani, J. Pharm. Biomed. Anal., 50, 107 (2009).
18. A. Sulkowska, J. Mol. Struct., 614, 227 (2002).
19. X. L. Jin, X. Wei, F. M. Qi, S. S. Yu, B. Zhou, S. Bai, Org. Biomol. Chem., 10, 3424 (2012).
20. T. Peters, Adv. Protein Chem., 37, 161 (1985).
21. M. S. Zaroog, S. Tayyab, Process Biochem., 47, 775 (2012).
22. S. R. Feroz, S. B. Mohamad, N. Bujang, S. N. A. Malek, S. Tayyab, J. Agric. Food. Chem., 60, 5899 (2012).
23. Y. Q. Wang, B. P. Tang, H. M. Zhang, Q. H. Zhou, G. C. Zhang, J. Photochem. Photobiol. B: Biol., 94, 183 (2009).
Review
For citations:
Wang Zh., Deng X., Hou J., Fan J. MOLECULAR SPECTROSCOPY EVIDENCE OF EFFECTIVE CONSTITUENTS IN THE BINDING OF Angelica sinensis TO BOVINE SERUM ALBUMIN: COMPARATIVE BINDING AND THERMODYNAMIC PROFILE. Zhurnal Prikladnoii Spektroskopii. 2018;85(6):939-945. (In Russ.)
Views: 256
Email this article 