RAMAN SPECTROSCOPY APPLICATION FOR ANALYSIS OF THE DISTRIBUTION OF CARBON NANOTUBES IN LIVING CELLS
Abstract
Suspensions of single- and double-walled carbon nanotubes of various lengths, as well as multi-walled carbon nanotubes, have been studied by Raman spectroscopy in combination with confocal microscopy. It is shown that the intensity of the G mode in the Raman spectra of the carbon nanotubes is directly proportional to the nanotube concentration, the exposure time, the power and focusing depth of the exciting radiation in the transparent sample under study. It is found that the Raman spectra of longer carbon nanotubes (~1 μm) are characterized by a higher intensity of the G mode compared with the short nanotubes (~250-450 nm). The dependences obtained have been used to determine the local intracellular concentration of the carbon nanotubes within the waist of the exciting laser beam in order to create a map of the distribution of the carbon nanotubes inside the cells.
About the Authors
L. N. Golubewa
Belarusian State University
Russian Federation
Russian Federation
M. V. Shuba
Research Institute for Nuclear Problems of Belarusian State University
Russian Federation
Russian Federation
M. V. Vasilieu
Belarusian State University
Russian Federation
Russian Federation
T. A. Kulahava
Belarusian State University
Russian Federation
Russian Federation
References
1. S. Reich, C. Thomsen, J. Maultzsch. Carbon Nanotubes: Basic Concepts and Physical Properties, Darmstadt, Wiley (2008) 3-4
2. D. Pantarotto, R. Singh, D. McCarthy, M. Erhardt, J. P. Briand, M. Prato, K. Kostarelos, A. Bianco. Angew. Chem. Int. Ed. Engl., 43, N 39 (2004) 5242-5246
3. Z. Liu, S. Tabakman, K. Welsher, H. Dai. Nano Res., 2, N 2 (2009) 85-120
4. S. Y. Madani, N. Naderi, O. Dissanayake, A. Tan, A. M. Seifalian. Int. J. Nanomed., 6 (2011) 2963-2979
5. A. M. Elhissi, W. Ahmed, I. U. Hassan, V. R. Dhanak, A. D’Emanuele. J. Drug Deliv. (2012) 2012:837327
6. H. K. Moon, S. H. Lee, H. C. Choi. ACS Nano, 3, N 11 (2009) 3707-3713
7. S. Jain, S. R. Singh, S. Pillai. J. Nanomed. Nanotechol., 3, N 5 (2012); doi:10.4172/2157-7439.1000140
8. V. M. Irurzun, M. P. Ruiz, D. E. Resasco. Carbon, 48, N 10 (2010) 2873-2881
9. C. Zavaleta, A. de la Zerda, Z. Liu, S. Keren, Z. Cheng, M. Schipper, X. Chen, H. Dai, S. S. Gambhir. Nano Lett., 8, N 9 (2008) 2800-2805
10. C. Lamprecht, N. Gierlinger, E. Heister, B. Unterauer, B. Plochberger, M. Brameshuber, P. Hinterdorfer, S. Hild, A. Ebner. J. Phys. Condens. Matter., 24, N 16 (2012)
11. C. Bertulli, H. J. Beeson, T. Hasan, Y. Y. Huang. Nanotechnol., 24, N 26 (2013) 265102
12. C. Fantini, A. Jorio, M. Souza, M. S. Strano, M. S. Dresselhaus, M. A. Pimenta. Phys. Rev. Lett., 93 (2004) 147406
13. Z. Liu, C. Davis, W. Cai, L. He, X. Chen, H. Dai. PNAS, 105 (2008) 1410-1415
14. Z. Liu, W. Cai, L. He, N. Nakayama, K. Chen, X. Sun, X. Chen, H. Dai. Nature Nanotechnol., 2, N 47 (2007) 47-52
15. D. A. Heller, S. Baik, T. E. Eurell, M. S. Strano. Adv. Mater., 17 (2005) 2793
16. J. W. Kang, F. T. Nguyen, N. Lue, R. R. Dasari, D. A. Heller. Nano Lett., 12 (2012) 6170-6174
17. B. D. Holt, K. N. Dahl, M. F. Islam. Small, 7 (2011) 2348-2355
18. I. V. Anoshkin, I. I. Nefedova, D. V. Lioubtchenko, I. S. Nefedov, A. V. Räisänen. Carbon, 116 (2017) 547-552
19. M. V. Shuba, A. G. Paddubskaya, P. P. Kuzhir, S. A. Maksimenko, V. Ksenevich, G. Niaura, D. Seliuta, I. Kašalynas, G. Valusis. Nanotechnol., 23 (2012) 495714
20. M. V. Shuba, A. Paddubskaya, P. P. Kuzhir, S. M. Maksimenko, E. Flahaut, V. Fierro, A. Celzard, G. Valusis. J. Phys. D, 50, 08LT01 (2017)
21. V. Neves, E. Heister, S. Costa, C. Tîlmaciu, E. Borowiak-Palen, C. E. Giusca, E. Flahaut, B. Soula, H. M. Coley, J. McFadden, S. R. P. Silva. Adv. Funct. Mater., 20, N 19 (2010) 3272-3279
22. A. S. Biris, E. I. Galanzha, Z. Li, M. Mahmood, Y. Xu, V. P. Zharov. J. Biomed. Opt., 14, N 2 (2009) 021006
Review
For citations:
Golubewa L.N., Shuba M.V., Vasilieu M.V., Kulahava T.A. RAMAN SPECTROSCOPY APPLICATION FOR ANALYSIS OF THE DISTRIBUTION OF CARBON NANOTUBES IN LIVING CELLS. Zhurnal Prikladnoii Spektroskopii. 2018;85(6):999-1005. (In Russ.)
Views: 295
Email this article 