MODIFICATION OF THE METHOD OF SPECTROPHOTOMETRIC DETERMINATION OF CHLOROPHYLL a IN THE SUSPENDED MATTER OF WATER BODIES
Abstract
This article describes a modification of the spectrophotometric method for the determination of chlorophyll a in seston (suspended matter), which is convenient for hydroecological monitoring. It is proposed to use nuclear filters, as they have a number of advantages over fiberglass and bulk filters. An important difference of our approach from the standard methods is the use of the short-term (up to 30 min) drying of nuclear filters with seston in a desiccator at a temperature of 50-55°C immediately after the suspended matter is deposited on the filter. A comparative analysis of the assessment of the chlorophyll and phaeopigments content by the standard method and the proposed modification is carried out using the laboratory cultures of algae (Euglena gracilis, Chlorella vulgaris) and plankton from a natural water body.
About the Authors
R. Z. Kovalevskaya
Belarusian State University
Belarus
Belarus
Minsk, 220030
H. A. Zhukava
Belarusian State University
Belarus
Belarus
Minsk, 220030
B. V. Adamovich
Belarusian State University
Belarus
Belarus
Minsk, 220030
References
1. Н. Г. Аверина, Л. Ф. Кабашникова, Н. В. Шалыго, И. Д. Волотовский. Весцi НАН Беларусі. Сер. біял. навук, 63, № 2 (2018) 248—256
2. J. Neveux, D. Delmas, J.C. Romano, P. Algarra, L. Ignatiades, A. Herbland, P. Morand, A. Neori, D. Bonin, J. Barbe, A. Sukenik, T. Berman. Marine Microbial Food Webs, 4, N 2 (1990) 217—238
3. F. A. Richards, T. G. Thompson. J. Marine Res., 11 (1952) 156—172
4. SCOR-UNESCO Working group N 17. Determination of Photosynthetic Pigments in Sea-water. Monographs on Oceanologic Methodology, Paris, UNESCO (1966) 9—18
5. ГОСТ 17.1.04.02.90. Вода. Методика спектрофотометрического определения хлорофилла-а, Москва (1999) 3—12
6. ISO 10260:1992, Water quality – Measurement of Biochemical Parameters – Spectrometric Determination of the Chlorophyll-a Concentration (1992) 1—6
7. СТБ 17.13.05-34-2014/ISO 10260:1992. Вызначэнне канцэнтрацыі хларафіла-а спектрафотаметрычным метадам, Мінск (2014) 1—9
8. C. J. Lorenzen. Limnol. Oceanogr., 12 (1967) 343—346
9. B. Moss. Limnol. Oceanogr., 12 (1967) 340—342
10. Л. Е. Сигарева. Методические вопросы изучения первичной продукции планктона внутренних водоемов, СПб, Гидрометеоиздат (1993) 75—85
11. R. J. Ritchie. Photosynthetica, 46, N 1 (2008) 115—126
12. A. F. H. Marker, E. A. Nusch, H. Rai, В. Riemann. Arch. Hydrobiol. Beih. Ergebn. Limnol., 14 (1980) 91—106
13. T. Pohlert. The Pairwise Multiple Comparison of Mean Ranks Package (PMCMR). R package (2014) 1—9
Review
For citations:
Kovalevskaya R.Z., Zhukava H.A., Adamovich B.V. MODIFICATION OF THE METHOD OF SPECTROPHOTOMETRIC DETERMINATION OF CHLOROPHYLL a IN THE SUSPENDED MATTER OF WATER BODIES. Zhurnal Prikladnoii Spektroskopii. 2020;87(1):84-91. (In Russ.)
Views: 334
Email this article 