A 4-HYDROXY-1,8-NAPHTHALIMIDE-BASED TURN-ON TWO-PHOTON FLUORESCENT PROBE FOR HYDROGEN POLYSULFIDE SENSING
Abstract
In order to quantitatively detect hydrogen polysulfides (H2Sn, n > 1) in biosystems, we propose a new two-photon turn-on fluorescent probe, named Np-Sn, based on the D-π-A skeleton two-photon fluorophore of 4-hydroxy-1,8-naphthalimide derivative and deprotection of 2-fluoro-5-nitrobenzoic ester by H2Sn. Np-Sn displayed a more than 80-fold enhancement towards H2Sn in 550 nm and high sensitivity with a detection limit as low as 33 nM. Additionally, the probe Np-Sn was further used for fluorescence imaging of H2Sn in living cells under two-photon excitation (820 nm), which showed a high-resolution imaging, thus demonstrating its practical application in biological systems for the study of physiological and pathological functions of H2Sn.
Supporting agencies: This work was supported by the National Natural Science Foundation of China (Grants 21605046) and the Natural Science Foundation of Hunan Province (Grants 2017JJ3060).
About the Authors
L. Zhou
College of Life Sciences and Chemistry, Hunan University of Technology; College of Food Science and Technology, Central South University of Forestry and Technology; Hunan University, State Key Laboratory for Chemo/Biosensing and Chemometrics
China
Hunan 412007; Hunan 410004; Hunan 410082
China
Hunan 412007; Hunan 410004; Hunan 410082
G. Yuan
College of Food Science and Technology, Central South University of Forestry and Technology
China
Hunan 410004
China
Hunan 410004
Sh. Hu
College of Life Sciences and Chemistry, Hunan University of Technology
China
Hunan 412007
China
Hunan 412007
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Review
For citations:
Zhou L., Yuan G., Hu Sh. A 4-HYDROXY-1,8-NAPHTHALIMIDE-BASED TURN-ON TWO-PHOTON FLUORESCENT PROBE FOR HYDROGEN POLYSULFIDE SENSING. Zhurnal Prikladnoii Spektroskopii. 2019;86(6):962-967.
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