SURFACE-ENHANCED RAMAN SCATTERING-BASED IMMUNOASSAY OF TWO CYTOKINES IN HUMAN GINGIVAL CREVICULAR FLUID OF PERIODONTAL DISEASE
Abstract
This study combined Raman technology and nanotechnology to detect periodontal inflammatory factors. We synthesized silver nanoparticles and made modifications to their surface to produce Ag NP-antibody probes. A sandwich immunoassay was developed for the sensitive detection of TNF-α and IL-1β. SERS intensity showed a good linear relationship with TNF-α and IL-1β concentrations in our study. The results of detection of these biomarkers in GCF indicate excellent specificity, high sensitivity, and great reproducibility of this immunoassay. Our immunoassay based on the SERS technique would facilitate clinical diagnoses of periodontal diseases.
Keywords
periodontal disease,
gingival crevicular fluid,
surface-enhanced Raman scattering,
cytokines,
inflammation
Supporting agencies: The work was performed with financial support from the National Key Basic Research and Development Program (grant No.2013CBA01700), National Nature Science Foundation of China (grant No. 81371149), and Chengdu Medical Research Project (No. 2018026).
About the Authors
H. Yang
Chengdu First People’s Hospital
China
Chengdu, 610041
China
Chengdu, 610041
M. L. Zhang
Chongqing Share dent Clinic
China
Chongqing, 400010
China
Chongqing, 400010
L. H. Yao
The First Affiliated Hospital of Zhengzhou University
China
Henan, 450000
China
Henan, 450000
M. Zhou
Stomatological Hospital of Chongqing Medical University
China
Chongqing, 400017
China
Chongqing, 400017
Q. Wang
National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University
China
China
State Key Laboratory of Oral Diseases
Chengdu, 610041
Yu Chen
National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University
China
China
State Key Laboratory of Oral Diseases
Chengdu, 610041
Y. Ding
National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University
China
China
State Key Laboratory of Oral Diseases
Chengdu, 610041
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Review
For citations:
Yang H., Zhang M.L., Yao L.H., Zhou M., Wang Q., Chen Yu., Ding Y. SURFACE-ENHANCED RAMAN SCATTERING-BASED IMMUNOASSAY OF TWO CYTOKINES IN HUMAN GINGIVAL CREVICULAR FLUID OF PERIODONTAL DISEASE. Zhurnal Prikladnoii Spektroskopii. 2019;86(6):968-975.
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