ASSESSMENT OF JOINT EFFECT OF IBUPROFEN AND CHOLESTEROL ON MICROVISCO-SITY AND ORDERING OF MODEL LIPID MEMBRANES BY TIME-RESOLVED MEASUREMENTS OF FLUORESCENCE ANISOTROPY DECAY

The time-resolved fluorescence anisotropy decay of perylene incorporated into the lipid L-α-dipalmitoylphosphatidylcholine (DPPC) membrane has been studied to evaluate the membranotropic action of non-steroidal anti-inflammatory substance ibuprofen and the joint effect of ibuprofen and cholesterol. The obtained values of rotation correlation times (j) and limiting anisotropy (r_∞) allow estimating independently the effects of these additives on microviscosity and ordering of model lipid membranes in different phase states. Ibuprofen is shown to decrease significantly the DPPC membrane microviscosity in the gel phase with no actual effect in the liquid-crystal phase. However, in both phases ibuprofen decreases the ordering of the lipid hydrophobic chains. When ibuprofen is embedded in a lipid membrane enriched with cholesterol, a pronounced additive effect is observed in the gel phase manifesting itself in substantial dilution and disordering of the lipid membrane by the components. In the liquid-crystal phase, ibuprofen causes leveling of the densifying and regulating effects of cholesterol.

разрешенное во времени затухание анизотропии флуоресценции, время вращательной корреляции, перилен, ибупрофен, холестерин, модельная липидная мембрана, time-resolved fluorescence anisotropy decay, rotation correlation time, perylene, ibuprofen, model lipid membrane, cholesterol

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