DETECTION OF DNA HYBRIDIZATION USING ZnS:Mn2+ NANOWIRES/SiO2 CORE/SHELL NANOCOMPOSITES AND Au NANOPARTICLES
Abstract
The ZnS:Mn2+ nanowires (NWs)/SiO2 nanocomposites and Au nanoparticles (NPs) were used for the first time as donors and acceptors to detect DNA hybridization basing on the mechanism of fluorescence resonance energy transfer (FRET). The ZnS:Mn2+ NWs/SiO2 core/shell nanocomposites with the average diameter of 25 nm were synthesized by the Stöber method. The Au NPs with the average diameter of 19 nm were prepared by the seed-mediated growth method. The fluorescence intensity of ZnS:Mn2+ NWs/SiO2-dsDNA-Au NPs conjugates decreased extremely compared with that of ZnS:Mn2+ NWs/SiO2-DNA conjugates, indicating that FRET occurred between the ZnS:Mn2+ NWs/SiO2 nanocomposites and Au NPs. When the target DNA sequence was added, the fluorescence intensity was restored. Thus, the selective hybridization of the probe DNA to the target DNA can be used for detecting the target DNA.
Keywords
Supporting agencies: This work was supported by the National Natural Science Foundation of China (No. 61705079, 61475063), the National Key Research and Development Program of China (Grant No. 2017YFF0108607).
About the Authors
Yue Zhou
Jilin Normal University
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Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, National Demonstration Center for Experimental Physics Education
Jian Cao
Jilin Normal University
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Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, National Demonstration Center for Experimental Physics Education
Lili Yang
Jilin Normal University
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Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, National Demonstration Center for Experimental Physics Education
Maobin Wei
Jilin Normal University
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Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, National Demonstration Center for Experimental Physics Education
Xiaoyan Liu
Jilin Normal University
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Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, National Demonstration Center for Experimental Physics Education
Qianyu Liu
Jilin Normal University
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Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, National Demonstration Center for Experimental Physics Education
Xin Li
Jilin Normal University
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Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, National Demonstration Center for Experimental Physics Education
Jinghai Yang
Jilin Normal University
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Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, National Demonstration Center for Experimental Physics Education
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Review
For citations:
Zhou Yu., Cao J., Yang L., Wei M., Liu X., Liu Q., Li X., Yang J. DETECTION OF DNA HYBRIDIZATION USING ZnS:Mn2+ NANOWIRES/SiO2 CORE/SHELL NANOCOMPOSITES AND Au NANOPARTICLES. Zhurnal Prikladnoii Spektroskopii. 2019;86(3):382-387.
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