EMISSION ANISOTROPY OF THE FLUORESCEIN COVALENTLY LINKED TO OLIGONUCLEOTIDES
Abstract
We studied the rotational depolarization of the fluorescence of molecular systems of covalently linked fluorescein and oligonucleotides. The dependence of the fluorescence anisotropy of two nucleic acids on the temperature-to-viscosity ratio was measured in buffer solutions with different glycerol concentrations. It was shown that the experimental data can be satisfactorily explained within the diffusion model of an elongated molecular top with internal rotation. It was found that the coefficient of the internal rotational diffusion in all the cases was 1.5-2 times larger than the corresponding coefficient for the rotation around the axis of the oligonucleotide.
About the Authors
A. P. Blokhin
B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
M. V. Kvach
Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
V. A. Povedailo
B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
V. V. Shmanai
Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
D. L. Yakovlev
B. I. Stepanov Institute of Physics, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
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Review
For citations:
Blokhin A.P., Kvach M.V., Povedailo V.A., Shmanai V.V., Yakovlev D.L. EMISSION ANISOTROPY OF THE FLUORESCEIN COVALENTLY LINKED TO OLIGONUCLEOTIDES. Zhurnal Prikladnoii Spektroskopii. 2017;84(1):26-32. (In Russ.)
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