STUDY OF MECHANISMS OF THYMOQUINONE ANTITUMOR ACTION USING RAMAN SPECTROSCOPY
Abstract
The mechanisms of 2-isopropyl-5-methyl-1,4-benzoquinone (thymoquinone) action on human larynx carcinoma cells of the HEp-2 line were studied using methods of fluorescence assay and Raman spectroscopy. It was found that thymoquinone has a more pronounced toxic effect compared to 1,4-benzoquinone and 2,3,5-trimethyl-1,4-benzoquinone. A decrease of the mitochondrial potential and the release of cytochrome c from the mitochondria are observed under the action of thymoquinone, which indicates the activation of apoptosis of the tumor cells along the mitochondrial-mediated pathway. The obtained results demonstrate the prospects of using Raman spectroscopy in the study of programmed cell death.
About the Authors
A. V. Vcherashniaya
Belarusian State University
Belarus
Minsk, 220030
Belarus
Minsk, 220030
I. V. Martinovich
Belarusian State University
Belarus
Minsk, 220030
Belarus
Minsk, 220030
G. G. Martinovich
Belarusian State University
Belarus
Minsk, 220030
Belarus
Minsk, 220030
O. I. Shadyro
Belarusian State University
Belarus
Minsk, 220030
Belarus
Minsk, 220030
S. N. Cherenkevich
Belarusian State University
Belarus
Minsk, 220030
Belarus
Minsk, 220030
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Review
For citations:
Vcherashniaya A.V., Martinovich I.V., Martinovich G.G., Shadyro O.I., Cherenkevich S.N. STUDY OF MECHANISMS OF THYMOQUINONE ANTITUMOR ACTION USING RAMAN SPECTROSCOPY. Zhurnal Prikladnoii Spektroskopii. 2020;87(3):488-492. (In Russ.)
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