STUDY OF THE INTERACTION OF CEFONICID SODIUM WITH BOVINE SERUM ALBUMIN BY FLUORESCENCE SPECTROSCOPY
Abstract
The reaction mechanism of cefonicid sodium with bovine serum albumin was investigated by traditional fluorescence spectroscopy and synchronous fluorescence spectroscopy. The results demonstrated that cefonicid sodium caused a strong fluorescence quenching of bovine serum albumin through a static quenching mechanism, during which the electrostatic force played the dominant role in this system, and the number of binding sites in the system was close to 1. It also showed that the primary binding site for cefonicid sodium was closer to tryptophan residues located in sub-hydrophobic domain IIA. Moreover, circular dichroism spectroscopy showed that the secondary structure of bovine serum albumin changed. The donor-to-acceptor distance r < 8 nm indicated that the static fluorescence quenching of bovine serum albumin was a non-radiation energy transfer process. The data obtained from Δl = 60 nm and lex= 295 nm indicated that synchronous fluorescence spectroscopy had higher sensitivity and accuracy compared to traditional fluorescence spectroscopy.
Keywords
спектроскопия тушения флуоресценции,
синхронная флуоресцентная спектроскопия,
абсорбционная спектроскопия ИК и видимого диапазона,
спектроскопия циркулярного дихроизма,
цефоницид натрия,
бычий сывороточный альбумин,
fluorescence quenching spectroscopy,
synchronous fluorescence spectroscopy,
UV-Vis absorption spectroscopy,
circular dichroism spectroscopy,
cefonicid sodium,
bovine serum albumin
About the Authors
Sh. -T. Duan
College of Chemistry & Environmental Science, Hebei University
Russian Federation
Russian Federation
B. -Sh. Liu
College of Chemistry & Environmental Science, Hebei University
Russian Federation
Russian Federation
T. -T. Li
College of Chemistry & Environmental Science, Hebei University
Russian Federation
Russian Federation
M. -M. Cui
College of Chemistry & Environmental Science, Hebei University
Russian Federation
Russian Federation
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Review
For citations:
Duan Sh.-., Liu B.-., Li T.-., Cui M.-. STUDY OF THE INTERACTION OF CEFONICID SODIUM WITH BOVINE SERUM ALBUMIN BY FLUORESCENCE SPECTROSCOPY. Zhurnal Prikladnoii Spektroskopii. 2017;84(3):410-418. (In Russ.)
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