METHOD FOR SEPARATION OF BLOOD VESSELS ON THE THREE-COLOR IMAGES OF BIOLOGICAL TISSUES
Abstract
A new technology has been developed to improve the visibility of blood vessels on the images of biological tissues based on the relation between image color components, scattering properties of the tissue and hemoglobin content in it. A statistical operator was presented to convert a three-color image of tissue into the parametric map objectively characterizing the concentration of hemoglobin in the tissue regardless of the illumination and shooting conditions. An algorithm for obtaining the conversion parameters for imaging systems with known spectral characteristics was presented. An image of a multilayer multiple-scattering medium modeling bronchial tissue was synthesized, and the efficiency of the proposed conversion was evaluated on its basis. It was shown that the conversion makes it possible to increase the contrast of blood vessels by almost two orders and significantly improve the clarity of the display of their borders, to almost completely eliminate the influence of background and non-uniform illumination of the medium in comparison with the original image.
Keywords
биологическая ткань,
кровеносные сосуды,
трехцветные изображения,
многократное рассеяние,
гемоглобиновый индекс,
локальный диаметр сосуда,
параметрические карты,
biological tissue,
blood vessels,
three-colored images,
multiple scattering,
hemoglobin index,
local vessel diameter,
parametric maps
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Review
For citations:
Lisenko S.A. METHOD FOR SEPARATION OF BLOOD VESSELS ON THE THREE-COLOR IMAGES OF BIOLOGICAL TISSUES. Zhurnal Prikladnoii Spektroskopii. 2017;84(3):419-427. (In Russ.)
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