SYNERGISTIC INTERACTIONS OF PHYTOCHEMICALS IN POLYHERBAL FORMULATION ENHANCE THE CHEMICAL TRANSFORMATIONS OF ACTIVE CONSTITUENTS
Abstract
Synergetic chemical interaction of various phytochemicals in Amruthotharam kashayam, a Polyherbal formulation, has been evaluated by different spectroscopic techniques. Comparative chemical profiling was done along with its ingredient plants by HPTLC analysis. Characterization of major chemical constituents was carried out by Fourier transform infra-red spectroscopy (FTIR) and tandem mass spectroscopic analyses (LC/MS-MS). The chromatographic profiling and spectrophotometric studies revealed that the chemistry of the finished formulation is different from that of individual plant extracts. FTIR analysis showed the spectral shift in the formulation when compared with corresponding transmittance in the ingredient plants. The synergetic chemical reaction during the process is evidenced by such specific spectral shift. The tandem mass spectroscopic studies also confirmed the chemical transformations happening during the preparation of Amruthotharam kashayam.
Keywords
Amruthotharam kashayam,
chemical transformation,
Fourier transform infra-red spectroscopy,
tandem mass spectroscopic analyses
Supporting agencies: The authors thank Navajbhai Ratan Tata Trust, Mumbai, for financial assistance.
About the Authors
C. T. Sulaiman
Phytochemistry division, Centre for Medicinal Plants Research
India
India
Arya Vaidya Sala, Kottakkal, Kerala
K. Anju
Depertment of Chemistry, KSM Devaswom Board College
India
Sasthamcotta, Kollam, Kerala
India
Sasthamcotta, Kollam, Kerala
E. M. Anandan
Product Development Department
India
Arya Vaidya Sala, Kottakkal, Kerala
India
Arya Vaidya Sala, Kottakkal, Kerala
I. Balachandran
Phytochemistry division, Centre for Medicinal Plants Research
India
Arya Vaidya Sala, Kottakkal, Kerala
India
Arya Vaidya Sala, Kottakkal, Kerala
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Review
For citations:
Sulaiman C.T., Anju K., Anandan E.M., Balachandran I. SYNERGISTIC INTERACTIONS OF PHYTOCHEMICALS IN POLYHERBAL FORMULATION ENHANCE THE CHEMICAL TRANSFORMATIONS OF ACTIVE CONSTITUENTS. Zhurnal Prikladnoii Spektroskopii. 2021;88(1):168(1)-168(6).
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