PLASMONIC SENSOR FOR DETECTION OF β-LACTAM ANTIBIOTICS BASED ON THE CONJUGATED ANTIBODY WITH GOLD NANOPARTICLES
Abstract
This study aims to detect β-lactam antibiotics using a conjugated antibody with gold nanoparticles (GNPs). For this purpose, the gold nanoparticles synthesized from Chinese lettuce leaf extract (as reductant) were used for the colorimetric detection of β-lactam antibiotics (such as ampicillin, amoxicillin, penicillin G, oxacillin, and carbenicillin). XRD, FTIR spectroscopy, TEM, and dynamic light scattering were utilized to detect the crystallinity, to identify functional groups involved in the synthesis of GNPs, and to measure the size of the GNPs; pH 8 and a concentration of 8.4 μg of antibody at 1 mL GNPs solution were selected as the best pH and concentration of antibody for the conjugation of antibody with GNPs. The maximum wavelengths of the colloidal GNPs, conjugation of antibody with GNPs, and detection of antibiotics (from 1 nM to 1 mM) with GNPs-PAb were recorded using a micro-volume spectrophotometer system. The results indicated that the localized surface plasmon resonance spectrometer absorption wavelength of GNPs red-shifted with increasing concentration of β-lactam antibiotics. With increasing concentration of ampicillin, penicillin G, and carbenicillin, the wavelength of maximum changed, and after saturation of antibiotics concentration, the curve reaches a plateau. This indicated that the antibody showed similar behavior in the detection of these antibiotics. But regarding amoxicillin, the saturation concentration is much higher, indicating that the antibody was more specific for its detection. In contrast, for oxacillin, saturation occurred very soon, which demonstrated that the antibody had an extremely low detection capability for this antibiotic. Finally, the results showed that the antibody was sensitive to 1 nM of the five β-lactam antibiotics studied.
Keywords
localized surface plasmon resonance spectrometer,
β-lactam antibiotics,
polyclonal antibody of β-lactam,
gold nanoparticles,
conjugation
Supporting agencies: This article is a part of a Ph.D. thesis approved at the University of Tabriz. This work was financially supported by Iran Nanotechnology Initiative Council (Grant code: 136472). The authors would like to thank Prof. Mohammad Javad Rasaee from Tarbiat Modares University, Dr. Seyed Mohammad Amini from Iran University of Medical Sciences, and Mr. Gholamreza Behrouzi, Mr. Babak Farshid, Miss Zahra Abolghasemi-Fakhri, and Mrs. Samira Tizchang for their very useful comments. We thank Rojan Azma Co. and its personnel for supporting research equipment. Finally, we thank Daneshmand Research & Development Institute and Mostazafan Foundation of Islamic Revolution.
About the Authors
M. Aghamirzaei
University of Tabriz
Islamic Republic of Iran
Tabriz
Islamic Republic of Iran
Tabriz
M. S. Khiabani
University of Tabriz
Islamic Republic of Iran
Tabriz
Islamic Republic of Iran
Tabriz
H. Hamishehkar
Drug Applied Research Center at Tabriz University of Medical Sciences
Islamic Republic of Iran
Tabriz
Islamic Republic of Iran
Tabriz
R. R. Mokarram
University of Tabriz
Islamic Republic of Iran
Tabriz
Islamic Republic of Iran
Tabriz
M. Amjadi
University of Tabriz
Islamic Republic of Iran
Tabriz
Islamic Republic of Iran
Tabriz
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Review
For citations:
Aghamirzaei M., Khiabani M.S., Hamishehkar H., Mokarram R.R., Amjadi M. PLASMONIC SENSOR FOR DETECTION OF β-LACTAM ANTIBIOTICS BASED ON THE CONJUGATED ANTIBODY WITH GOLD NANOPARTICLES. Zhurnal Prikladnoii Spektroskopii. 2021;88(1):174(1)-174(10).
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