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Validated uv spectrophotometric methods for the determination of anticancer drug erlotinib hydrochloride in bulk and tablet formulations
Abstract
Two different simple, accurate, and precise UV spectrophotometric methods have been developed for the estimation of erlotinib hydrochloride in bulk and tablet forms by the zero-order (method I) and the zeroorder AUC (method II). The drug was dissolved in a phosphate buffer solution (pH 7.4), and at 228.20 nm the zero-order (method I) and at 224.20–230.20 nm the zero-order AUC (method II) were developed. For both methods, erlotinib has linearity in the concentration range 10–70 µg/mL, with a correlation coefficient R2 > 0.99. Both methods showed good reproducibility and recovery with %RSD less than 2 and an accuracy of 98.54–101.39 and 98.08–99.83%, respectively. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.16, 3.51, and 21.41, 64.90 µg/mL, respectively, for both methods.
Supporting agencies: The authors are thankful to the R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur (M.S.), India for providing the required facilities to carry out this research work. The authors are thankful to MSN Laboratories Private Limited Unit-II, Telangana, for providing erlotinib hydrochloride as a gift sample.
About the Authors
R. D. Rathod
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research
India
India
Shirpur 425405
L. D. Rathod
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research
India
India
Shirpur 425405
A. B. Mundada
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research
India
India
Shirpur 425405
H. M. Patel
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research
India
India
Shirpur 425405
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Review
For citations:
Rathod R.D., Rathod L.D., Mundada A.B., Patel H.M. Validated uv spectrophotometric methods for the determination of anticancer drug erlotinib hydrochloride in bulk and tablet formulations. Zhurnal Prikladnoii Spektroskopii. 2021;88(4):667-671.
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