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400 MHz Nuclear Magnetic Resonance Metabolite Profiles of Jaw Cysts, Jaw Abscesses, Saliva, and Urine Obtained by Massive D2O Dilution of Samples
Abstract
The metabolite profiles of various cysts and abscesses outside the jaw area have been investigated by magnetic resonance spectroscopy (MRS) using water-suppression techniques. However, metabolite profiles of jaw cysts, jaw abscesses, saliva, and urine have not yet been obtained by MRS using massive D2O dilution of samples. This study was aimed at obtaining metabolite profiles of these bio-fluids by MRS without water suppression. In total, 37 jaw cysts, 19 jaw abscesses, 10 saliva and 10 urine samples were collected. Dilutions were prepared by adding 0.06 mL of each sample to 0.94 mL D2O. Single-pulse experiments were performed using a 400 MHz nuclear magnetic resonance (NMR) spectrometer. High-resolution metabolite profiles were obtained for most jaw abscesses (13), some jaw cysts (8), all saliva, and all urine samples. Except for one jaw cyst, the remaining samples showed a broad envelope containing various metabolites. However, high-resolution spectra for these cystic fluid samples were obtained through 40% dilution with H2O. In conclusion, to the best of our knowledge, metabolite profiles of jaw cysts, abscesses, saliva, and urine by massive D2O dilution were first presented here. The presented approach does not require water suppression and uses a relatively low frequency NMR. It is also a simple and novel method of eliminating radiation damping, reducing experimental time, and allowing relaxation measurements.
Supporting agencies: We thank Professor Murat Aydemir for his courtesy during NMR measurements. We thank Barbara Spathelf, PhD, from Edanz (https://jp.edanz.com/ac) for editing a draft of this manuscript.
About the Authors
U. N. Yilmaz
University of Dicle
Turkey
Turkey
Department of Oral-Maxillofacial Surgery, Faculty of Dentistry,
Diyarbakır
B. D. Yilmaz
University of Dicle
Turkey
Turkey
Department of Prosthodontics, Faculty of Dentistry,
Diyarbakır
M. Z. Köylü
University of Dicle
Turkey
Turkey
Department of Physics, Faculty of Science,
Diyarbakır
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Review
For citations:
Yilmaz U.N., Yilmaz B.D., Köylü M.Z. 400 MHz Nuclear Magnetic Resonance Metabolite Profiles of Jaw Cysts, Jaw Abscesses, Saliva, and Urine Obtained by Massive D2O Dilution of Samples. Zhurnal Prikladnoii Spektroskopii. 2022;89(6):845-851.
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