Pure Electronic Transition and Homogeneousity of Luminophore from Chemi- and Bioluminescence Spectra

Abstract

It is shown that, despite the complex mechanism of formation of the excited state, the chemi- and bioluminescence spectra, like ordinary diffuse fluorescence spectra, contain information about the magnitude of the purely electronic (vertical) transition of emitting molecules and the degree of homogeneity of their ensemble. The purely electronic transition, as in the case of ordinary diffuse luminescence spectra, is located significantly, almost a half-width of the band, shorter than the maximum of the fluorescence spectrum. An example of the separation of overlapping spectra using the position of the short-wavelength pure-electronic transition is given.