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Fluorescence Studies on Flavonoid Diglycosides and Corresponding Deglycosylated Monoglycosides
Abstract
A fluorescence quantitative detection method suitable for high-throughput screening of rhamnosidase catalytic activity on naringin was established. Detection of prunin at λex/λem = 360/450 nm was based on the fluorescence difference after heating under alkaline conditions. Detection of naringin at λex/λem = 325/400 nm was based on the difference in fluorescence in phosphate buffer. The method is suitable for prescreening many samples. A similar method can detect the fluorescence of hesperidin and hesperitin-7-o-glucoside, which provides a reference for the rapid detection of diglycosides and corresponding deglycosylated monoglycosides.
Supporting agencies: We greatly appreciate useful discussion from Qi Chen, Sun Jiang, and Zhang Yichun, appreciate the funding support from the National Natural Science Foundation of China (No. U1805235), the Science and technology project of Xiamen City, Fujian Province, China (No. 3502Z20203058), and the Central financial award project of the modern agricultural industrial park in Pinghe County, Fujian Province, China (No. PH2020) for this project.
About the Authors
H. Li
College of Ocean Food and Biological Engineering at Jimei University
China
China
Xiamen
W. Li
College of Ocean Food and Biological Engineering at Jimei University
China
China
Xiamen
G. Huang
College of Ocean Food and Biological Engineering at Jimei University
China
China
Xiamen
L. Li
College of Ocean Food and Biological Engineering at Jimei University
China
China
Xiamen
H. Ni
College of Ocean Food and Biological Engineering at Jimei University
China
China
Xiamen
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Review
For citations:
Li H., Li W., Huang G., Li L., Ni H. Fluorescence Studies on Flavonoid Diglycosides and Corresponding Deglycosylated Monoglycosides. Zhurnal Prikladnoii Spektroskopii. 2023;90(1):128. (In Russ.)
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