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Development and Validation of UV Spectrophotometric Method for Determination of Prazosin Hydrochloride
Abstract
This work was aimed at creating a new, fast, and accurate UV spectrophotometric method for quantifying prazosin hydrochloride in pure and tablet dosage forms. A phosphate buffer solution of pH 6 was used as a diluent. The highest absorbance of prazosin hydrochloride was measured at 247 nm, and the linearity ranged from 2 to 8 µg/mL. The regression equation for prazosin hydrochloride was y= 0.087x + 0.236, with a correlation value 0.987. The percentage of recovery ranged from 99.6 to 101%. The relative standard deviation for intraday precision and interday precision was determined to be less than 2. The LOD and LOQ of prazosin hydrochloride were determined to be 0.0375 and 0.113 µg/mL, respectively. International Council for Harmonisation criteria validated the spectrometric technique and was suitable for routine quantitative measurement of prazosin hydrochloride in pure and tablet dosage forms.
About the Authors
C. S. Bharathi
GITAM (Deemed to be University)
Russian Federation
Russian Federation
Department of Pharmaceutical Analysis, GITAM School of Pharmacy,
Visakhapatnam, Andhra Pradesh (State)
R. Sundararajan
GITAM (Deemed to be University)
Russian Federation
Russian Federation
Department of Pharmaceutical Analysis, GITAM School of Pharmacy,
Visakhapatnam, Andhra Pradesh (State)
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Review
For citations:
Bharathi C.S., Sundararajan R. Development and Validation of UV Spectrophotometric Method for Determination of Prazosin Hydrochloride. Zhurnal Prikladnoii Spektroskopii. 2024;91(3):468.
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