FTIR SPECTROSCOPY OF PROTEIN ISOLATES OF SALT-TOLERANT SOYBEAN MUTANTS
Abstract
The effect of salinity on the conformation of proteins of four salt-tolerant M2 generation mutants of soybean plants (S04-05/150-2, S04-05/150-8, S04-05/150-106, and S04-05/150-114) was investigated using Fourier transform infrared (FTIR) spectroscopy. Salinity is one of the important abiotic stress factors that limits growth and productivity of plants. The mutants belonging to the M2 generation were determined as tolerant to 90 mM NaCl. The relative contents of a-helix, b-sheet, turn, and irregular conformations for the soybean protein isolates were determined depending on the analysis of the amide I region. The comparison of the secondary structures of soybean proteins of the mutants with those of the control group indicated that the a-helix structure percentage was diminished while b-turn and disordered structures were increased as a result of the salt stress.
About the Authors
S. . Akyuz
Istanbul Kultur University
Russian Federation
Russian Federation
T. . Akyuz
Istanbul Kultur University
Russian Federation
Russian Federation
O. . Celik
Istanbul Kultur University
Russian Federation
Russian Federation
C. . Atak
Istanbul Kultur University
Russian Federation
Russian Federation
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Review
For citations:
Akyuz S., Akyuz T., Celik O., Atak C. FTIR SPECTROSCOPY OF PROTEIN ISOLATES OF SALT-TOLERANT SOYBEAN MUTANTS. Zhurnal Prikladnoii Spektroskopii. 2017;84(6):942-946. (In Russ.)
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