OPTICAL METHODS FOR THE ANALYSIS OF THE TEMOPORFIN PHOTOSENSIBILIZER DISTRIBUTION BETWEEN SERUM PROTEINS AND METHYL-b-CYCLODEXTRIN NANO-CARRIERS IN THE BLOOD SERUM

Different optical methods for the analysis of the process of temoporfin photosensitizer distribution between supramolecular nanosized inclusion complexes based on b-cyclodextrins and blood serum proteins were considered. We compared the application possibilities of the methods based on induced circular dichroism, fluorescence anisotropy, and variability of the fluorescence excitation spectra shape of the photosensitizer with such traditional methods as gel-chromatography and ultracentrifugation. The possibility of using the photosensitizer optical characteristics for the analysis of both equilibrium and kinetic processes of photosensitizer distribution in blood was shown. The main advantages and limitations of the studied approaches in in vitro experiments were described.

темопорфин, b-циклодекстрины, комплексы включения, сыворотка крови, поляризация флуоресценции, круговой дихроизм, полоса Соре, temoporfin, b-cyclodextrins, inclusion complexes, blood serum, fluorescence anisotropy, circular dichroism, Soret band

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