Fluorescence characteristics of HOECHST 33258 complexes with singlestranded and double-stranded polyribonucleotides at various ionic strength of the solution

Comparative study on the interaction of Hoechst 33258 (H33258) with single-stranded (ss-) polyribonucleotides poly(rA) and poly(rU) and double-stranded (ds-) poly(rA)-poly(rU) has been carried out with the method of fluorescence spectroscopy. Groove-binding compound H33258, being specific ligand for NA ds-structure, was revealed to bind to ss-RNA. It was revealed that H33258 shows higher preference to poly(rA)-poly(rU), formed due to the hybridization, than to ss-poly(rA) and poly(rU). Based on the fluorescence spectra the binding curves of H33258 to ss- and ds- polyribonucleotides were constructed and the values of binding constant K and number of bases per binding site n were determined. The obtained data elucidate that the binding constant of H33258 to ss-poly(rA) is 4-5 times higher, as compared to ss-poly(rU) one. It was also shown that H33258 affinity to poly(rA)-poly(rU) and ss-poly(rA) depends on the ionic strength of the solution, while for ss-poly(rU) such a dependence is not found.

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