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Derivative Spectrophotometric Methods for Estimation of Seratrodast in Bulk and in Formulation
Abstract
Seratrodast is a 1,4-benzoquinone derivative belonging to the class of thromboxane A2 (TXA2) receptor antagonists and is employed for prophylactic management of asthma. The present report describes the validation of quick, simple, sensitive, and cost-effective zero-order, first-order, and second-order derivative spectrophotometric methods for the estimation of seratrodast in bulk and in its marketed tablet formulation. Exhaustive spectrophotometric analysis of the drug was carried out using a total of 23 parametric variations, which were evaluated in acetonitrile. Three selected method variants were assessed for their stability, indicating potential regarding stress-degraded solutions of the drug. The developed methods were validated with respect to linearity, accuracy, precision, and robustness. Excellent linearity was observed in the concentration range of 5.0–50.0 μg/mL with correlation coefficients above 0.999. The limits of detection were found to range from 0.54 to 0.70 µg/mL, and quantitation limits ranged from 1.66 to 2.14 μg/mL for the proposed method variants. The proposed methods were also employed for the assay of seratrodast in its marketed tablet formulation, and good recoveries ranging from 96.23 to 98.68% were obtained.
Keywords
seratrodast,
spectrophotometric,
method validation,
derivative spectrophotometry,
first derivative,
second derivative,
analysis
Supporting agencies: This work was supported by the University Grants Commission (UGC), New Delhi, India (Grant No. 36/N/296).
About the Authors
Kanade Krushna Devrao
Panjab University
India
India
University Institute of Pharmaceutical Sciences, UGC Center of Advanced Study
Chandigarh
Alka Bali
Panjab University
India
India
University Institute of Pharmaceutical Sciences, UGC Center of Advanced Study
Chandigarh
Priyanshi Sharma
Panjab University
India
India
University Institute of Pharmaceutical Sciences, UGC Center of Advanced Study
Chandigarh
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For citations:
Devrao K.K., Bali A., Sharma P. Derivative Spectrophotometric Methods for Estimation of Seratrodast in Bulk and in Formulation. Zhurnal Prikladnoii Spektroskopii. 2026;93(1):143/1-143/8.
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