RAPID DETECTION OF TETRODOTOXIN USING SURFACE-ENHANCED RAMAN SPECTROSCOPY AND Fe3O4/SiO2/Au GOLD/MAGNETIC NANOPARTICLES
Abstract
Fe3O4 nanoparticles were first modified with tetraethoxylsilane to form Fe3O4/SiO2 nanoparticles, followed by the addition of 3-aminopropyltriethoxysilane and 3-thiolpropyltriethoxysilane to introduce -NH2 and -SH groups to the surface of Fe3O4/SiO2 nanoparticles. Gold nanoparticles were further assembled on the surface of Fe3O4/SiO2 via the electrostatic adsorption of -NH2 and the Au-S bond to produce stable core-shell Fe3O4/SiO2/Au gold/magnetic nanoparticles. These Fe3O4/SiO2/Au gold/magnetic nanoparticles were characterized by a variety of techniques such as transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX), and afterwards conjugated with tetrodotoxin antibodies (Ab) and used as a Raman active substrate (Fe3O4/SiO2/Au-Ab) with Rhodamine B (RhB)-labeled tetrodotoxin antibody as a Raman reporter (Ab-RhB). Upon mixing these reagents with tetrodotoxin (TTX), a sandwich complex [Fe3O4/SiO2/Au-Ab···TTX···Ab-RhB] was generated due to the specific antibody-antigen interactions. The immunocomplex was subsequently separated by an externally applied magnetic source and concentrated into a pellet point, where the laser interrogation of the pellet produced a strong signal characteristic of RhB. The logarithmic intensity of the signal was found to be proportional to the concentration of TTX with a limit of detection of 0.01µg/mL and a detection linearity range of ~0.01-0.5 µg/mL. The established method eliminates the complicated procedures of traditional centrifuging, column separation, and incubation and achieves a rapid detection of tetrodotoxin with improved detection sensitivity.
About the Authors
J. . Neng
Ocean College, Zhejiang University of Technology
Russian Federation
Russian Federation
X. . Wang
Ocean College, Zhejiang University of Technology
Russian Federation
Russian Federation
K. . Jia
Ocean College, Zhejiang University of Technology
Russian Federation
Russian Federation
P. . Sun
Ocean College, Zhejiang University of Technology
Russian Federation
Russian Federation
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Review
For citations:
Neng J., Wang X., Jia K., Sun P. RAPID DETECTION OF TETRODOTOXIN USING SURFACE-ENHANCED RAMAN SPECTROSCOPY AND Fe3O4/SiO2/Au GOLD/MAGNETIC NANOPARTICLES. Zhurnal Prikladnoii Spektroskopii. 2018;85(1):169(1)-169(7). (In Russ.)
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