INTERACTION OF EMODIN AND ITS DERIVATIVE FRANGULIN-A WITH BOVINE SERUM ALBUMIN AND CALF THYMUS DNA
Abstract
The interaction between emodin, frangulin-A (obtained from Ventilago leiocarpa Benth) with bovine serum albumin (BSA) and calf thymus DNA (ct-DNA) has been investigated. The fluorescence spectrum and molecular docking methods are used to investigate the interaction between emodin, frangulin-A, and BSA. The UV spectrum revealed interaction between emodin, frangulin-A, and ct-DNA. The fluorescence spectrum confirmed that the compounds of emodin-BSA and frangulin-A-BSA were formed showing fluorescence quenching and a decrease of the maximum emission wavelength. The simulated molecular docking showed stable combinations of emodin-BSA and frangulin-A-BSA. Thus, molecular interactions mainly occur due to hydrophobic forces and hydrogen bonds. According to the UV spectrum, emodin and frangulin-A interacted with ct-DNA via electrostatic interaction.
Supporting agencies: This work was financially supported by the Natural Science Foundation of Guangxi Province (2016GXNSFAA380092, 2017GXNSFBA198214), Guangxi Key Laboratory of traditional Chinese Medicine Quality Standards (201506, 201601), and Guangxi Science and Technology Project (AD18216002).
About the Authors
X.-X. Hu
Guangxi Institute of Traditional Medical and Pharmaceutical Sciences
China
Nanning, 530022
China
Nanning, 530022
Zh.-F. Huang
Guangxi Institute of Traditional Medical and Pharmaceutical Sciences
China
Nanning, 530022
China
Nanning, 530022
G.-Sh. Lu
Guangxi Institute of Traditional Medical and Pharmaceutical Sciences; Guangxi Key Laboratory of Traditional Chinese Medicine Quality Stardands
China
Nanning, 530022
China
Nanning, 530022
J.-Y. Huang
Guangxi Institute of Traditional Medical and Pharmaceutical Sciences
China
Nanning, 530022
China
Nanning, 530022
X. Tan
Guangxi Institute of Traditional Medical and Pharmaceutical Sciences
China
Nanning, 530022
China
Nanning, 530022
G.-T. Huang
Reproductive Medicine Center, Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region
China
530004, Nanning
China
530004, Nanning
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Review
For citations:
Hu X., Huang Zh., Lu G., Huang J., Tan X., Huang G. INTERACTION OF EMODIN AND ITS DERIVATIVE FRANGULIN-A WITH BOVINE SERUM ALBUMIN AND CALF THYMUS DNA. Zhurnal Prikladnoii Spektroskopii. 2020;87(1):56-63.
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