ESTIMATING JOINT CARTILAGE THICKNESS ON AN ANIMAL MODEL ex vivo USING DIFFUSE REFLECTANCE SPECTROSCOPY
Abstract
A diffuse reflectance visible light spectroscopy method has been developed to estimate bovine cartilage thickness in real time. The system consists of a miniature UV-VIS spectrometer, a halogen tungsten light source, and an optical fiber probe including two 400 μm diameter fibers with a center to center separation of 1.2 mm was used to acquire the spectra. A total of four patellae obtained from bovine just after sacrifice. In the study, ten cattle patella cartilage samples were prepared in a cylindrical shape and thinned by a 200 µm step. Spectra were acquired from the 123 cartilage samples. Cartilage samples were divided into training and validation groups. A correlation between the thickness of the cartilage samples and the absorption spectra was obtained using the data of the training group. The relative thickness of the cartilage was estimated with an average error of 15% in the validation group using the correlation. Diffuse reflectance spectroscopy has the potential to estimate the thickness of cartilage lesions during arthroscopic evaluation of knee cartilages.
Supporting agencies: This study was supported by The Scientific and Technological Research Council of Turkey Grant (grant 115S474) and partly by the Akdeniz University Scientific Research Grant.
About the Authors
A. Sircan-Kucuksayan
Alanya Alaaddin Keykubat University
Turkey
Department of Biophysics, Faculty of Medicine, Antalya
Turkey
Department of Biophysics, Faculty of Medicine, Antalya
M. Canpolat
Akdeniz University
Turkey
Biomedical Optics Research Unit, Department of Biophysics, Faculty of Medicine, Antalya
Turkey
Biomedical Optics Research Unit, Department of Biophysics, Faculty of Medicine, Antalya
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Review
For citations:
Sircan-Kucuksayan A., Canpolat M. ESTIMATING JOINT CARTILAGE THICKNESS ON AN ANIMAL MODEL ex vivo USING DIFFUSE REFLECTANCE SPECTROSCOPY. Zhurnal Prikladnoii Spektroskopii. 2019;86(4):569-574.
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