DEVELOPMENT AND VALIDATION OF AN UV SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF THIAMPHENICOL IN DOSAGE FORM
Abstract
A novel UV spectrophotometric method has been developed for the determination of thiamphenicol in a solid dosage form. The spectrophotometric detection was carried out at an absorption maximum wavelength of 224 nm using water as solvent. The method was validated for specificity, linearity, precision, accuracy, robustness, and limit of detection (LOD) and limit of quantitation (LOQ). The detector response for thiamphenicol was linear in the selected concentration range of 5 to 25 µg/ml with a correlation coefficient of 0.9975. The precision (RSD) among six sample preparations was 0.65 and 1.50 % for analysts 1 and 2, respectively. The average recovery was 99.91 ± 0.65%. The LOD and LOQ were respectively 0.59 and 1.99 µg/ml. The results demonstrated that the excipients in thiamphenicol soft capsules did not interfere with the spectrophotometric determination of the drug. Using the Youden and Steiner approach, the method proved to be robust. The proposed method can be conveniently employed for routine analysis of thiamphenicol in bulk drug and soft capsules.
Supporting agencies: The authors thank Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP), Federal University of Ceará (UFC), and UFC School of Pharmacy for the financial support.
About the Authors
Y. A. Martins
Universidade Federal do Ceará
Brazil
Departamento de Farmácia, Faculdade de Farmácia, Odontologia e Enfermagem, Rua Capitão Francisco Pedro, 1210, CEP 60430-370, Fortaleza, CE
Brazil
Departamento de Farmácia, Faculdade de Farmácia, Odontologia e Enfermagem, Rua Capitão Francisco Pedro, 1210, CEP 60430-370, Fortaleza, CE
C. L. C. G. De Oliveira
Universidade Federal do Ceará
Brazil
Departamento de Farmácia, Faculdade de Farmácia, Odontologia e Enfermagem, Rua Capitão Francisco Pedro, 1210, CEP 60430-370, Fortaleza, CE
Brazil
Departamento de Farmácia, Faculdade de Farmácia, Odontologia e Enfermagem, Rua Capitão Francisco Pedro, 1210, CEP 60430-370, Fortaleza, CE
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Review
For citations:
Martins Y.A., De Oliveira C. DEVELOPMENT AND VALIDATION OF AN UV SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF THIAMPHENICOL IN DOSAGE FORM. Zhurnal Prikladnoii Spektroskopii. 2019;86(4):575-581.
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