STUDY ON REGULATORY EFFECT OF 2-HEXADECENAL ON NEUTROPHILS BY THE CHEMILUMINESCENT METHOD
Abstract
The effect of 2-hexadecenal on the formation of reactive oxygen and chlorine species (ROCS) in neutrophils stimulated to phagocytosis has been determined by chemiluminescent method. It has been established that at low concentrations this aldehyde demonstrates a priming effect on the cells, enhancing ROCS production, and at higher concentrations – significantly suppresses this process. Comparison the results of chemiluminescent and fluorescent analysis of the cell characteristics suggests that 2-hexadecenal is a signaling molecule, which exhibits the properties of the neutrophil function regulator by modifying the processes of intracellular signaling associated with changes in ROCS production, cytoskeleton reorganization, increase in unbound calcium ions levels in cytoplasm, reduction of mitochondrial membrane potential. It also induces apoptosis.
About the Authors
N. V. Amaegberi
Belarusian State University
Belarus
4 Nezavisimosti Prosp., Minsk, 220030
Belarus
4 Nezavisimosti Prosp., Minsk, 220030
G. N. Semenkova
Belarusian State University
Belarus
4 Nezavisimosti Prosp., Minsk, 220030
Belarus
4 Nezavisimosti Prosp., Minsk, 220030
A. G. Lisovskaya
Belarusian State University; University of Notre Dame, USA
Belarus
Belarus
4 Nezavisimosti Prosp., Minsk, 220030, Belarus;
Notre Dame Radiation Laboratory, University of Notre Dame, Notre Dame, Indiana, 46556 USA
S. S. Gusakova
Belarusian State University
Belarus
4 Nezavisimosti Prosp., Minsk, 220030
Belarus
4 Nezavisimosti Prosp., Minsk, 220030
V. A. Prokasheva
Belarusian State University
Belarus
4 Nezavisimosti Prosp., Minsk, 220030
Belarus
4 Nezavisimosti Prosp., Minsk, 220030
O. I. Shadyro
Belarusian State University
Belarus
4 Nezavisimosti Prosp., Minsk, 220030
Belarus
4 Nezavisimosti Prosp., Minsk, 220030
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Review
For citations:
Amaegberi N.V., Semenkova G.N., Lisovskaya A.G., Gusakova S.S., Prokasheva V.A., Shadyro O.I. STUDY ON REGULATORY EFFECT OF 2-HEXADECENAL ON NEUTROPHILS BY THE CHEMILUMINESCENT METHOD. Zhurnal Prikladnoii Spektroskopii. 2019;86(4):582-589. (In Russ.)
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