OPTIMIZATION AND VALIDATION OF THE SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF DEXMEDETOMIDINE HYDROCHLORIDE IN PURE AND DOSAGE FORMS
Abstract
Four simple, precise, low-cost, sensitive, and diversely applicable UV-Vis spectrophotometric methods have been developed for the assay of dexmedetomidine hydrochloride in pure and pharmaceutical dosage forms. The methods are based on the oxidation of the drug by N-bromosuccinimide (NBS) (excess) at room temperature and estimating the amount of unconsumed NBS by amaranth dye at λmax = 530 nm (method A), safranin dye at λmax = 530 nm (method B), aniline blue at λmax = 610 nm (method C), or rhodamine B at λmax = 560 nm (method D). Regression analysis of Beer-Lambert’s plots proves excellent correlation in the concentration ranges 2-9, 4-11, 2-10, and 1.2-3.5 μg/ml for methods A, B, C, and D, respectively. The apparent molar absorptivity, Sandell’s sensitivity, and detection and quantification limits are calculated. The proposed methods can be applied to drug formulation and recommended for routine analysis in quality control laboratories. Statistical comparison of the results with the reference method shows excellent agreement.
About the Authors
K. P. Roopa
Sapthagiri college of Engineering
India
India
Department of chemistry
Bangalore
K. Basavaiah
University of Mysore
India
Department of Studies in Chemistry, Manasagangothri Mysore
India
Department of Studies in Chemistry, Manasagangothri Mysore
B. K. Jayanna
B.N.M. Institute of technology
India
India
Department of chemistry
Bangalore
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Review
For citations:
Roopa K.P., Basavaiah K., Jayanna B.K. OPTIMIZATION AND VALIDATION OF THE SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF DEXMEDETOMIDINE HYDROCHLORIDE IN PURE AND DOSAGE FORMS. Zhurnal Prikladnoii Spektroskopii. 2019;86(4):670(1)-670(8).
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