EFFECT OF THE HYPOGLYCEMIC AGENT GLICLAZIDE ON THE GASTRIC DIGESTIVE FUNCTION: BINDING MECHANISM BETWEEN GLICLAZIDE AND PEPSIN
Abstract
Herein, the interaction between gliclazide (GCZ) and pepsin (PEP) was explored under simulated physiological conditions by multiple spectroscopy methods and molecular docking. The results showed that a new complex of 1:1 was formed between GCZ and PEP, thereby quenching the endogenous fluorescence of PEP. The addition of GCZ changed the conformation of PEP and increased the α-helix content in PEP from 20.16% to 21.13%. Using the binding constant Ka of the reaction between GCZ and PEP and the number of binding sites n, the binding rate formulas of GCZ and PEP were deduced. It was estimated that when the patient takes 40 mg of GCZ, the PEP in the gastric juice will be reduced by 96.69%. That meant taking GCZ will seriously affect the patient's digestive function. The results of molecular docking indicated that the GCZ binding site was located in the active center of PEP. The interaction of the two was driven by electrostatic attraction and hydrogen bonding forces.
Supporting agencies: The authors gratefully acknowledge the financial support of the National Natural Science Foundation of China (No. 21375032).
About the Authors
L.-H. Ma
Hebei University, National Chemistry Experimental Teaching Demonstration Center Hebei University, College of Chemistry & Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province
China
Baoding 071002, Hebei Province
China
Baoding 071002, Hebei Province
B.-Sh. Liu
Hebei University, National Chemistry Experimental Teaching Demonstration Center Hebei University, College of Chemistry & Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province
China
Baoding 071002, Hebei Province
China
Baoding 071002, Hebei Province
G. Bian
Hebei University, National Chemistry Experimental Teaching Demonstration Center Hebei University, College of Chemistry & Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province
China
Baoding 071002, Hebei Province
China
Baoding 071002, Hebei Province
Ch.-D. Wang
Hebei University, National Chemistry Experimental Teaching Demonstration Center Hebei University, College of Chemistry & Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province
China
Baoding 071002, Hebei Province
China
Baoding 071002, Hebei Province
H.-C. Zhang
Hebei University, National Chemistry Experimental Teaching Demonstration Center Hebei University, College of Chemistry & Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province
China
Baoding 071002, Hebei Province
China
Baoding 071002, Hebei Province
X. Cheng
Hebei University, National Chemistry Experimental Teaching Demonstration Center Hebei University, College of Chemistry & Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province
China
Baoding 071002, Hebei Province
China
Baoding 071002, Hebei Province
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Review
For citations:
Ma L., Liu B., Bian G., Wang Ch., Zhang H., Cheng X. EFFECT OF THE HYPOGLYCEMIC AGENT GLICLAZIDE ON THE GASTRIC DIGESTIVE FUNCTION: BINDING MECHANISM BETWEEN GLICLAZIDE AND PEPSIN. Zhurnal Prikladnoii Spektroskopii. 2019;86(4):671(1)-671(8).
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