DEVELOPMENT AND VALIDATION OF THE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF MENTHOL
Abstract
The main objective of the present study is a quantitative determination of menthol using a simple, sensitive, and reproducible method of reacting menthol with an aromatic aldehyde (salicylaldehyde) in an acidic medium. A linear response was observed between the concentration and the absorbance in the range 0.02–3 mg/5mL possessing a correlation coefficient greater than 0.999 and exhibiting good recovery values (99.54–100.40%). The wavelength of the maximum absorbance (λmax) was measured at 498 nm immediately after the reaction and subsequently within 1 h. The proposed method was validated according to the ICH guidelines for parameters including linearity, precision, accuracy, range, stability, and robustness. Hence the developed method can be used in the analysis of menthol in drug formulations.
About the Authors
H. Kushwah
Institute of Nuclear Medicine & Allied Sciences, DRDO
India
Delhi
India
Delhi
T. Hans
Indian Institute of Technology
India
Gandhinagar, Gujarat
India
Gandhinagar, Gujarat
M. Chauhan
Delhi Institute of Pharmaceutical Sciences and Research
India
Delhi
India
Delhi
G. Mittal
Institute of Nuclear Medicine & Allied Sciences, DRDO
India
Delhi
India
Delhi
N. Sandal
Institute of Nuclear Medicine & Allied Sciences, DRDO
India
Delhi
India
Delhi
References
1. R. Eccles, J. Pharm. Pharmacol., 46, 618–630 (1994).
2. G. P. P. Kamatou, I. Vermaak, A. M. Viljoen, B. M. Lawrence, Phytochemistry, 96, 15–25 (2013).
3. N. Galeotti, L. Di Cesare Mannelli, G. Mazzanti, A. Bartolini, C. Ghelardini, Neurosci. Lett., 322, 145–148 (2002).
4. K. Shaikh, S. Patil, J. Pharm. Bioallied Sci., 2, 360–364 (2010).
5. L. Karuza, K. Folivarski, J. Pharm. Biomed. Anal., 15, 419–422 (1996).
6. M. Ligor, B. X. Buszewski, J. Chromatogr. A, 847, 161–169 (1999).
7. J. S. Valdez, D. K. Martin, M. Mayersohn, J. Chromatogr. B, Biomed. Sci. Appl., 729, 163–171 (1999).
8. Y. Tsuruta, Y. Date, K. Kohashi, Anal. Sci., 73, 411–414 (1991).
9. S. A. Haut, M. T. Core, J. Liq. Chromatogr., 4, 1869–1874 (1981).
10. K. Hamasaki, K. Kato, T. Watanabe, Y. Yoshimura, H. Nakazawa, A. Yamamoto, A. Matsunaga, J. Pharm. Biomed. Anal., 16, 1275–1280 (1998).
11. Y. Kasai, M. Watanabe, N. Harada, Chirality, 15, 295–299 (2003).
12. H. Auterhoff, H. Bertram, Arch. Pharm., 577, 3–8 (1973).
13. S. Kale, J. Chromatogr. Sep. Tech., 8, 3902157–3907064 (2017).
14. M. Maleque, M.R. Hasan, F. Hossen, S. Safi, J. Pharm. Anal., 2, 454–457(2012).
15. ICH, ICH Topic Q2 (R1). Validation of Analytical Procedures: Text and Methodology, Int. Conf. Harmon, 1994, 17 (2005).
16. S. K. Dangwal, Ind. Health, 18, N 4, 187–193 (1980).
17. A. Anastasia-Sandu, S. Bȋrzu, I. Diţu, L. Bulgariu, Bull. Polytech. Inst. Iasi, Sect. Chem. Chem. Eng., 71–80 (2013).
Review
For citations:
Kushwah H., Hans T., Chauhan M., Mittal G., Sandal N. DEVELOPMENT AND VALIDATION OF THE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF MENTHOL. Zhurnal Prikladnoii Spektroskopii. 2020;87(3):509(1)-509(5).
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