Hemoglobin water solutions scattering indicatrix

Abstract

Human hemoglobin water solution was studied by a multi-angle static light scattering method. The sickle shape of erythrocytes was explained, in particular, by the ability of hemoglobin molecules to aggregation. The experimental data were processed by the methods of Debye, asymmetry, and Zimm. As a result of the work, the values of scattering particles weight were obtained and molecule size was estimated. It was assumed that the structure of the hemoglobin molecule had changed, and the molecule had acquired an elongated shape. The data obtained by the Zimm method are in the best agreement with the data obtained before. The large sizes of scattering particles obtained by the methods of asymmetry and Debye are possibly related to the fact that the dissociation of hemoglobin is a reversible dynamic process, the equilibrium of which depends on the protein concentration/