VARIABILITY ESTIMATION OF THE PROTEIN AND PHENOL TOTAL CONTENT IN HONEY USING FRONT FACE FLUORESCENCE SPECTROSCOPY COUPLED WITH MCR-ALS ANALYSIS

It has been explored to what extent the ratio of the two main fluorophores in honey, originating from proteins and phenolic compounds, change between the honey extraction stage and packaging in the jars. Fluorescence spectroscopy combined with multivariate curve resolution alternating least squares (MCR-ALS) was used to determine the ratio of the spectral components originating from phenolics and proteins (Ph/Pr)_fl, as a ratiometric indicator of variability in selected Lime tree (Tilia L.) honey samples. Spectrophotometric quantification of phenols and proteins in the honey samples was also performed and their ratio (Ph/Pr)_sp calculated. The values of the (Ph/Pr)_fl ratio and honey protein content after packaging depended on the quality of homogenization before packing in jars. Colorimetric and fluorometric results for phenols and proteins were in compliance. The examples are the values 3.34, 3.30 and 9, 3.14 for (Ph/Pr)_fl and corresponding values 2.64, 2.18 and 12.75, 2.31 for (Ph/Pr)_sp, in all pairs, the first value presenting the sample after extraction and the second value the sample after packing in jars. Both methods show that in the former case there is no change in the phenol/protein ratio, and in the latter case the ratio decreased.

мед, флуоресцентная спектроскопия, алгоритм разрешения многомерных кривых методом чередующихся наименьших квадратов, количественное определение фенола и белка, показатель отношения, мелиссопалинологический анализ, honey, fluorescence spectroscopy, multivariate curve resolution alternating least squares, phenol and protein quantification, ratio metric indicator, melissopalynological analysis

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